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| | ==Crystal Structure of the Yeast Tom70== | | ==Crystal Structure of the Yeast Tom70== |
| - | <StructureSection load='2gw1' size='340' side='right' caption='[[2gw1]], [[Resolution|resolution]] 3.00Å' scene=''> | + | <StructureSection load='2gw1' size='340' side='right'caption='[[2gw1]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2gw1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GW1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2gw1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GW1 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TOM70, MAS70, OMP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TOM70, MAS70, OMP1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gw1 OCA], [http://pdbe.org/2gw1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gw1 RCSB], [http://www.ebi.ac.uk/pdbsum/2gw1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2gw1 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gw1 OCA], [https://pdbe.org/2gw1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gw1 RCSB], [https://www.ebi.ac.uk/pdbsum/2gw1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gw1 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TOM70_YEAST TOM70_YEAST]] Component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.<ref>PMID:11054285</ref> | + | [[https://www.uniprot.org/uniprot/TOM70_YEAST TOM70_YEAST]] Component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.<ref>PMID:11054285</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Atcc 18824]] | | [[Category: Atcc 18824]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Sha, B]] | | [[Category: Sha, B]] |
| | [[Category: Wu, Y]] | | [[Category: Wu, Y]] |
| | [[Category: Protein transport]] | | [[Category: Protein transport]] |
| | [[Category: Tpr]] | | [[Category: Tpr]] |
| Structural highlights
Function
[TOM70_YEAST] Component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
A majority of the proteins targeted to the mitochondria are transported through the translocase of the outer membrane (TOM) complex. Tom70 is a major surface receptor for mitochondrial protein precursors in the TOM complex. To investigate how Tom70 receives the mitochondrial protein precursors, we have determined the crystal structure of yeast Tom70p to 3.0 A. Tom70p forms a homodimer in the crystal. Each subunit consists primarily of tetratricopeptide repeat (TPR) motifs, which are organized into a right-handed superhelix. The TPR motifs in the N-terminal domain of Tom70p form a peptide-binding groove for the C-terminal EEVD motif of Hsp70, whereas the C-terminal domain of Tom70p contains a large pocket that may be the binding site for mitochondrial precursors. The crystal structure of Tom70p provides insights into the mechanisms of precursor transport across the mitochondrion's outer membrane.
Crystal structure of yeast mitochondrial outer membrane translocon member Tom70p.,Wu Y, Sha B Nat Struct Mol Biol. 2006 Jul;13(7):589-93. Epub 2006 Jun 11. PMID:16767096[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Brix J, Ziegler GA, Dietmeier K, Schneider-Mergener J, Schulz GE, Pfanner N. The mitochondrial import receptor Tom70: identification of a 25 kDa core domain with a specific binding site for preproteins. J Mol Biol. 2000 Nov 3;303(4):479-88. PMID:11054285 doi:http://dx.doi.org/10.1006/jmbi.2000.4120
- ↑ Wu Y, Sha B. Crystal structure of yeast mitochondrial outer membrane translocon member Tom70p. Nat Struct Mol Biol. 2006 Jul;13(7):589-93. Epub 2006 Jun 11. PMID:16767096 doi:http://dx.doi.org/10.1038/nsmb1106
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