6i60
From Proteopedia
(Difference between revisions)
| Line 3: | Line 3: | ||
<StructureSection load='6i60' size='340' side='right'caption='[[6i60]], [[Resolution|resolution]] 2.74Å' scene=''> | <StructureSection load='6i60' size='340' side='right'caption='[[6i60]], [[Resolution|resolution]] 2.74Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6i60]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[6i60]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dict6 Dict6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I60 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AE3:2-(2-ETHOXYETHOXY)ETHANOL'>AE3</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AE3:2-(2-ETHOXYETHOXY)ETHANOL'>AE3</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DICTH_0289 ([ | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DICTH_0289 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=309799 DICT6])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i60 OCA], [https://pdbe.org/6i60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i60 RCSB], [https://www.ebi.ac.uk/pdbsum/6i60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i60 ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | alpha-l-Rhamnosidases are catalysts of industrial tremendous interest, but their uses are still somewhat limited by their poor thermal stabilities and selectivities. The thermophilic DtRha from Dictyoglomus thermophilum was cloned, and the recombinant protein was easily purified to homogeneity to afford 4.5 mg/L culture of biocatalyst. Michaelis-Menten parameters demonstrated it to be fully specific for alpha-l-rhamnose. Most significantly, DtRha demonstrated to have a stronger preference for alpha(1 --> 2) linkage rather than alpha(1 --> 6) linkage when removing rhamnosyl moiety from natural flavonoids. This selectivity was fully explained by the difference of binding of the corresponding substrates in the active site of the protein. | ||
| + | |||
| + | Biochemical Characterization of the alpha-l-Rhamnosidase DtRha from Dictyoglomus thermophilum: Application to the Selective Derhamnosylation of Natural Flavonoids.,Guillotin L, Kim H, Traore Y, Moreau P, Lafite P, Coquoin V, Nuccio S, de Vaumas R, Daniellou R ACS Omega. 2019 Jan 24;4(1):1916-1922. doi: 10.1021/acsomega.8b03186. eCollection, 2019 Jan 31. PMID:31459445<ref>PMID:31459445</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6i60" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 10:27, 18 March 2021
Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum
| |||||||||||
