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1fou

From Proteopedia

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Revision as of 07:06, 24 March 2021

CONNECTOR PROTEIN FROM BACTERIOPHAGE PHI29

Structural highlights

1fou is a 12 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[VG10_BPPH2] This protein is located between the head and the tail of this bacteriophage.

Publication Abstract from PubMed

Motors generating mechanical force, powered by the hydrolysis of ATP, translocate double-stranded DNA into preformed capsids (proheads) of bacterial viruses and certain animal viruses. Here we describe the motor that packages the double-stranded DNA of the Bacillus subtilis bacteriophage phi29 into a precursor capsid. We determined the structure of the head-tail connector--the central component of the phi29 DNA packaging motor--to 3.2 A resolution by means of X-ray crystallography. We then fitted the connector into the electron densities of the prohead and of the partially packaged prohead as determined using cryo-electron microscopy and image reconstruction analysis. Our results suggest that the prohead plus dodecameric connector, prohead RNA, viral ATPase and DNA comprise a rotary motor with the head-prohead RNA-ATPase complex acting as a stator, the DNA acting as a spindle, and the connector as a ball-race. The helical nature of the DNA converts the rotary action of the connector into translation of the DNA.

Structure of the bacteriophage phi29 DNA packaging motor.,Simpson AA, Tao Y, Leiman PG, Badasso MO, He Y, Jardine PJ, Olson NH, Morais MC, Grimes S, Anderson DL, Baker TS, Rossmann MG Nature. 2000 Dec 7;408(6813):745-50. PMID:11130079^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Simpson AA, Tao Y, Leiman PG, Badasso MO, He Y, Jardine PJ, Olson NH, Morais MC, Grimes S, Anderson DL, Baker TS, Rossmann MG. Structure of the bacteriophage phi29 DNA packaging motor. Nature. 2000 Dec 7;408(6813):745-50. PMID:11130079 doi:10.1038/35047129

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fou"

@@ Line 3: / Line 3: @@
 <StructureSection load='1fou' size='340' side='right'caption='[[1fou]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1fou]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FOU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FOU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1fou]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FOU FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fou OCA], [http://pdbe.org/1fou PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fou RCSB], [http://www.ebi.ac.uk/pdbsum/1fou PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fou ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fou OCA], [https://pdbe.org/1fou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fou RCSB], [https://www.ebi.ac.uk/pdbsum/1fou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fou ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/VG10_BPPH2 VG10_BPPH2]] This protein is located between the head and the tail of this bacteriophage.
+[[https://www.uniprot.org/uniprot/VG10_BPPH2 VG10_BPPH2]] This protein is located between the head and the tail of this bacteriophage.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

1fou

From Proteopedia

Revision as of 07:06, 24 March 2021

CONNECTOR PROTEIN FROM BACTERIOPHAGE PHI29

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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