1dzz

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[[Image:1dzz.jpg|left|200px]]
[[Image:1dzz.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1dzz |SIZE=350|CAPTION= <scene name='initialview01'>1dzz</scene>, resolution 1.92&Aring;
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The line below this paragraph, containing "STRUCTURE_1dzz", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=ACT:Active+Center+Definded+By+The+Zn+Ion+And+The+Four+Zn+Coo+...'>ACT</scene>, <scene name='pdbsite=MUT:Mutation+Site'>MUT</scene> and <scene name='pdbsite=PBS:The+Substrate+Phosphate+Binding+Site+Near+The+Active+Cen+...'>PBS</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1dzz| PDB=1dzz | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dzz OCA], [http://www.ebi.ac.uk/pdbsum/1dzz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1dzz RCSB]</span>
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}}
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'''L-FUCULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI MUTANT Y113F'''
'''L-FUCULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI MUTANT Y113F'''
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[[Category: Joerger, A C.]]
[[Category: Joerger, A C.]]
[[Category: Schulz, G E.]]
[[Category: Schulz, G E.]]
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[[Category: aldolase (class ii)]]
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[[Category: Bacterial l-fucose metabolism]]
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[[Category: bacterial l-fucose metabolism]]
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[[Category: Cleavage of l-fuculose-1-phosphate to dihydroxyacetonephosphate and l-lactaldehyde]]
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[[Category: cleavage of l-fuculose-1-phosphate to dihydroxyacetonephosphate and l-lactaldehyde]]
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[[Category: Mutant structure]]
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[[Category: mutant structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:29:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:52:20 2008''
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Revision as of 11:29, 2 May 2008

Image:1dzz.jpg

Template:STRUCTURE 1dzz

L-FUCULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI MUTANT Y113F


Overview

Previous analyses established the structures of unligated L-fuculose 1-phosphate aldolase and of the enzyme ligated with an inhibitor mimicking the substrate dihydroxyacetone phosphate. These data allowed us to suggest a catalytic mechanism. On the basis of this proposal, numerous mutations were now introduced at the active center and tested with respect to their catalytic rates and their product distributions. For several mutants, the structures were determined. The results demonstrate the catalytic importance of some particular residues in defined conformations and in the mobile C-terminal chain end. Moreover, they led to a modification of the proposed mechanism. The effect of some mutations on enantioselectivity and on the ratio of diastereomer formation indicates clearly the binding site of the aldehyde moiety in relation to the other substrate dihydroxyacetone phosphate.

About this Structure

1DZZ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Catalytic action of fuculose 1-phosphate aldolase (class II) as derived from structure-directed mutagenesis., Joerger AC, Gosse C, Fessner WD, Schulz GE, Biochemistry. 2000 May 23;39(20):6033-41. PMID:10821675 Page seeded by OCA on Fri May 2 14:29:25 2008

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OCA

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