This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1e0k
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1e0k.jpg|left|200px]] | [[Image:1e0k.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1e0k", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1e0k| PDB=1e0k | SCENE= }} | |
| - | + | ||
| - | | | + | |
| - | }} | + | |
'''GP4D HELICASE FROM PHAGE T7''' | '''GP4D HELICASE FROM PHAGE T7''' | ||
| Line 29: | Line 26: | ||
[[Category: Singleton, M R.]] | [[Category: Singleton, M R.]] | ||
[[Category: Wigley, D B.]] | [[Category: Wigley, D B.]] | ||
| - | [[Category: | + | [[Category: Atpase]] |
| - | [[Category: | + | [[Category: Dna replication]] |
| - | [[Category: | + | [[Category: Helicase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:30:39 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:30, 2 May 2008
GP4D HELICASE FROM PHAGE T7
Overview
We have determined the crystal structure of an active, hexameric fragment of the gene 4 helicase from bacteriophage T7. The structure reveals how subunit contacts stabilize the hexamer. Deviation from expected six-fold symmetry of the hexamer indicates that the structure is of an intermediate on the catalytic pathway. The structural consequences of the asymmetry suggest a "binding change" mechanism to explain how cooperative binding and hydrolysis of nucleotides are coupled to conformational changes in the ring that most likely accompany duplex unwinding. The structure of a complex with a nonhydrolyzable ATP analog provides additional evidence for this hypothesis, with only four of the six possible nucleotide binding sites being occupied in this conformation of the hexamer. This model suggests a mechanism for DNA translocation.
About this Structure
1E0K is a Single protein structure of sequence from Enterobacteria phage t7. Full crystallographic information is available from OCA.
Reference
Crystal structure of T7 gene 4 ring helicase indicates a mechanism for sequential hydrolysis of nucleotides., Singleton MR, Sawaya MR, Ellenberger T, Wigley DB, Cell. 2000 Jun 9;101(6):589-600. PMID:10892646 Page seeded by OCA on Fri May 2 14:30:39 2008
