1e2u
From Proteopedia
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'''LOW TEMPERATURE STUCTURE OF HYBRID CLUSTER PROTEIN FROM DESULFOVIBRIO VULGARIS TO 1.6A''' | '''LOW TEMPERATURE STUCTURE OF HYBRID CLUSTER PROTEIN FROM DESULFOVIBRIO VULGARIS TO 1.6A''' | ||
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[[Category: Hagen, W R.]] | [[Category: Hagen, W R.]] | ||
[[Category: Lindley, P F.]] | [[Category: Lindley, P F.]] | ||
| - | [[Category: | + | [[Category: Cubane]] |
| - | [[Category: | + | [[Category: Fuscoredoxin]] |
| - | [[Category: | + | [[Category: Hybrid cluster]] |
| - | [[Category: | + | [[Category: Iron sulfur]] |
| - | [[Category: | + | [[Category: Prismane]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:35:27 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:35, 2 May 2008
LOW TEMPERATURE STUCTURE OF HYBRID CLUSTER PROTEIN FROM DESULFOVIBRIO VULGARIS TO 1.6A
Overview
The three-dimensional structure of the hybrid cluster protein from Desulfovibrio vulgaris (Hildenborough) has been determined at 1.6 A resolution using synchrotron X-ray radiation. The protein can be divided into three domains: an N-terminal mainly alpha-helical domain and two similar domains comprising a central beta-sheet flanked by alpha-helices. The protein contains two 4Fe clusters with an edge-to-edge distance of 10.9 A. Four cysteine residues at the N-terminus of the protein are ligands to the iron atoms of a conventional [4Fe-4S] cubane cluster. The second cluster has an unusual asymmetric structure and has been named the hybrid cluster to reflect the variety of protein ligands, namely two mu-sulfido bridges, two mu(2)-oxo bridges, and a further disordered bridging ligand. Anomalous differences in data collected at 1.488 A and close to the iron edge at 1.743 A have been used to confirm the identity of the metal and sulfur atoms. The hybrid cluster is buried in the center of the protein, but is accessible through a large hydrophobic cavity that runs the length of domain 3. Hydrophobic channels have previously been identified as access routes to the active centers in redox enzymes with gaseous substrates. The hybrid cluster is also accessible by a hydrophilic channel. The [4Fe-4S] cubane cluster is close to an indentation on the surface of the protein and can also be approached on the opposite side by a long solvent channel. At the present time, neither the significance of these channels nor, indeed, the function of the hybrid cluster protein is known.
About this Structure
1E2U is a Single protein structure of sequence from Desulfovibrio vulgaris. Full crystallographic information is available from OCA.
Reference
Hybrid-cluster protein (HCP) from Desulfovibrio vulgaris (Hildenborough) at 1.6 A resolution., Cooper SJ, Garner CD, Hagen WR, Lindley PF, Bailey S, Biochemistry. 2000 Dec 12;39(49):15044-54. PMID:11106482 Page seeded by OCA on Fri May 2 14:35:27 2008
