1a52

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Revision as of 13:49, 12 November 2007

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spinqualitylabelsall models 1a52, resolution 2.8Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

ESTROGEN RECEPTOR ALPHA LIGAND-BINDING DOMAIN COMPLEXED TO ESTRADIOL

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The 2.8-A crystal structure of the complex formed by estradiol and the, human estrogen receptor-alpha ligand binding domain (hERalphaLBD) is, described and compared with the recently reported structure of the, progesterone complex of the human progesterone receptor ligand binding, domain, as well as with similar structures of steroid/nuclear receptor, LBDs solved elsewhere. The hormone-bound hERalphaLBD forms a distinctly, different and probably more physiologically important dimer interface than, its progesterone counterpart. A comparison of the specificity determinants, of hormone binding reveals a common structural theme of mutually supported, van der Waals and hydrogen-bonded interactions involving highly conserved, residues. The previously suggested mechanism by which the estrogen, receptor distinguishes estradiol's unique 3-hydroxy group from the 3-keto, function of most other steroids is now described in atomic detail. Mapping, of mutagenesis results points to a coactivator-binding surface that, includes the region around the "signature sequence" as well as helix 12, where the ligand-dependent conformation of the activation function 2 core, is similar in all previously solved steroid/nuclear receptor LBDs. A, peculiar crystal packing event displaces helix 12 in the hERalphaLBD, reported here, suggesting a higher degree of dynamic variability than, expected for this critical substructure.

Disease

Known diseases associated with this structure: Atherosclerosis, susceptibility to OMIM:[133430], Breast cancer OMIM:[133430], Estrogen resistance OMIM:[133430], HDL response to hormone replacement, augmented OMIM:[133430], Migraine, susceptibility to OMIM:[133430], Myocardial infarction, susceptibility to OMIM:[133430]

About this Structure

1A52 is a Single protein structure of sequence from Homo sapiens with EST and AU as ligands. The following page contains interesting information on the relation of 1A52 with [Estrogen Receptor]. Full crystallographic information is available from OCA.

Reference

Crystallographic comparison of the estrogen and progesterone receptor's ligand binding domains., Tanenbaum DM, Wang Y, Williams SP, Sigler PB, Proc Natl Acad Sci U S A. 1998 May 26;95(11):5998-6003. PMID:9600906

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