1e3t
From Proteopedia
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| - | | | + | {{STRUCTURE_1e3t| PDB=1e3t | SCENE= }} |
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'''SOLUTION STRUCTURE OF THE NADP(H) BINDING COMPONENT (DIII) OF PROTON-TRANSLOCATING TRANSHYDROGENASE FROM RHODOSPIRILLUM RUBRUM''' | '''SOLUTION STRUCTURE OF THE NADP(H) BINDING COMPONENT (DIII) OF PROTON-TRANSLOCATING TRANSHYDROGENASE FROM RHODOSPIRILLUM RUBRUM''' | ||
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==Reference== | ==Reference== | ||
Solution structure of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase from Rhodospirillum rubrum., Jeeves M, Smith KJ, Quirk PG, Cotton NP, Jackson JB, Biochim Biophys Acta. 2000 Aug 15;1459(2-3):248-57. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11004437 11004437] | Solution structure of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase from Rhodospirillum rubrum., Jeeves M, Smith KJ, Quirk PG, Cotton NP, Jackson JB, Biochim Biophys Acta. 2000 Aug 15;1459(2-3):248-57. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11004437 11004437] | ||
| - | [[Category: NAD(P)(+) transhydrogenase (B-specific)]] | ||
[[Category: Rhodospirillum rubrum]] | [[Category: Rhodospirillum rubrum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Quirk, P G.]] | [[Category: Quirk, P G.]] | ||
[[Category: Smith, K J.]] | [[Category: Smith, K J.]] | ||
| - | [[Category: | + | [[Category: Membrane protein]] |
| - | [[Category: | + | [[Category: Nmr structure]] |
| - | [[Category: | + | [[Category: Nucleotide binding]] |
| - | [[Category: | + | [[Category: Proton translocation]] |
| - | [[Category: | + | [[Category: Transhydrogenase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:37:36 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:37, 2 May 2008
SOLUTION STRUCTURE OF THE NADP(H) BINDING COMPONENT (DIII) OF PROTON-TRANSLOCATING TRANSHYDROGENASE FROM RHODOSPIRILLUM RUBRUM
Overview
Transhydrogenase is a proton pump found in the membranes of bacteria and animal mitochondria. The solution structure of the expressed, 21.5 kDa, NADP(H)-binding component (dIII) of transhydrogenase from Rhodospirillum rubrum has been solved by NMR methods. This is the first description of the structure of dIII from a bacterial source. The protein adopts a Rossmann fold: an open, twisted, parallel beta-sheet, flanked by helices. However, the binding of NADP(+) to dIII is profoundly different to that seen in other Rossmann structures, in that its orientation is reversed: the adenosine moiety interacts with the first betaalphabetaalphabeta motif, and the nicotinamide with the second. Features in the structure that might be responsible for changes in nucleotide-binding affinity during catalysis, and for interaction with other components of the enzyme, are identified. The results are compared with the recently determined, high-resolution crystal structures of human and bovine dIII which also show the reversed nucleotide orientation.
About this Structure
1E3T is a Single protein structure of sequence from Rhodospirillum rubrum. Full crystallographic information is available from OCA.
Reference
Solution structure of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase from Rhodospirillum rubrum., Jeeves M, Smith KJ, Quirk PG, Cotton NP, Jackson JB, Biochim Biophys Acta. 2000 Aug 15;1459(2-3):248-57. PMID:11004437 Page seeded by OCA on Fri May 2 14:37:36 2008
