6v6z

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==Crystal structure of N-(4-((4-methoxy-N-(2,2,2-trifluoroethyl)phenyl)sulfonamido)isoquinolin-1-yl)-N-((4-methoxyphenyl)sulfonyl)glycine bound to human Keap1 Kelch domain==
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==Crystal structure of N-[(4-methoxyphenyl)sulfonyl]-N-(4-{[(4-methoxyphenyl)sulfonyl]amino}naphthalen-1-yl)glycine bound to human Keap1 Kelch domain==
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<StructureSection load='6v6z' size='340' side='right'caption='[[6v6z]]' scene=''>
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<StructureSection load='6v6z' size='340' side='right'caption='[[6v6z]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V6Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V6Z FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6v6z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V6Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6V6Z FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v6z OCA], [http://pdbe.org/6v6z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v6z RCSB], [http://www.ebi.ac.uk/pdbsum/6v6z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v6z ProSAT]</span></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTD:DITHIANE+DIOL'>DTD</scene>, <scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=Q5Y:N-[(4-methoxyphenyl)sulfonyl]-N-(4-{[(4-methoxyphenyl)sulfonyl]amino}naphthalen-1-yl)glycine'>Q5Y</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KEAP1, INRF2, KIAA0132, KLHL19 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6v6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v6z OCA], [https://pdbe.org/6v6z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6v6z RCSB], [https://www.ebi.ac.uk/pdbsum/6v6z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6v6z ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The oxidative stress response, gated by the protein-protein interaction of KEAP1 and NRF2, has garnered significant interest in the past decade. Misregulation in this pathway has been implicated in disease states such as multiple sclerosis, rheumatoid arthritis, and diabetic chronic wounds. Many of the known activators of NRF2 are electrophilic in nature and may operate through several biological pathways rather than solely through the activation of the oxidative stress response. Recently, our lab has reported a nonelectrophilic, monoacidic, naphthalene-based NRF2 activator which exhibited good potency in vitro. Herein, we report a detailed structure-activity relationship of naphthalene-based NRF2 activators, an X-ray crystal structure of our monoacidic KEAP1 inhibitor, and identification of an underexplored area of the NRF2 binding pocket of KEAP1.
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Synthesis and Evaluation of Noncovalent Naphthalene-Based KEAP1-NRF2 Inhibitors.,Lazzara PR, Jain AD, Maldonado AC, Richardson B, Skowron KJ, David BP, Siddiqui Z, Ratia KM, Moore TW ACS Med Chem Lett. 2020 Feb 19;11(4):521-527. doi:, 10.1021/acsmedchemlett.9b00631. eCollection 2020 Apr 9. PMID:32292559<ref>PMID:32292559</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6v6z" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Kelch-like protein 3D structures|Kelch-like protein 3D structures]]
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Ankireddy A]]
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[[Category: Ankireddy, A]]
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[[Category: David BP]]
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[[Category: David, B P]]
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[[Category: Dye K]]
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[[Category: Dye, K]]
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[[Category: Lazzara PR]]
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[[Category: Lazzara, P R]]
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[[Category: Moore TW]]
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[[Category: Moore, T W]]
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[[Category: Ratia KM]]
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[[Category: Ratia, K M]]
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[[Category: Reddy SP]]
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[[Category: Reddy, S P]]
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[[Category: Richardson BG]]
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[[Category: Richardson, B G]]
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[[Category: Keap1]]
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[[Category: Nrf2 activator]]
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[[Category: Protein binding-inhibitor complex]]
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[[Category: Protein-protein interaction inhibitor]]

Revision as of 07:04, 7 April 2021

Crystal structure of N-[(4-methoxyphenyl)sulfonyl]-N-(4-{[(4-methoxyphenyl)sulfonyl]amino}naphthalen-1-yl)glycine bound to human Keap1 Kelch domain

PDB ID 6v6z

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