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| | ==Solution structure of the alternative conformation of XCL1/Lymphotactin== | | ==Solution structure of the alternative conformation of XCL1/Lymphotactin== |
| - | <StructureSection load='2jp1' size='340' side='right' caption='[[2jp1]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2jp1' size='340' side='right'caption='[[2jp1]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2jp1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JP1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2jp1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JP1 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">XCL1, LTN, SCYC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">XCL1, LTN, SCYC1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jp1 OCA], [http://pdbe.org/2jp1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jp1 RCSB], [http://www.ebi.ac.uk/pdbsum/2jp1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jp1 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jp1 OCA], [https://pdbe.org/2jp1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jp1 RCSB], [https://www.ebi.ac.uk/pdbsum/2jp1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jp1 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/XCL1_HUMAN XCL1_HUMAN]] Chemotactic activity for lymphocytes but not for monocytes or neutrophils. | + | [[https://www.uniprot.org/uniprot/XCL1_HUMAN XCL1_HUMAN]] Chemotactic activity for lymphocytes but not for monocytes or neutrophils. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Human]] | | [[Category: Human]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Peterson, F C]] | | [[Category: Peterson, F C]] |
| | [[Category: Tuinstra, R L]] | | [[Category: Tuinstra, R L]] |
| Structural highlights
Function
[XCL1_HUMAN] Chemotactic activity for lymphocytes but not for monocytes or neutrophils.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Proteins often have multiple functional states, which might not always be accommodated by a single fold. Lymphotactin (Ltn) adopts two distinct structures in equilibrium, one corresponding to the canonical chemokine fold consisting of a monomeric three-stranded beta-sheet and carboxyl-terminal helix. The second Ltn structure solved by NMR reveals a dimeric all-beta-sheet arrangement with no similarity to other known proteins. In physiological solution conditions, both structures are significantly populated and interconvert rapidly. Interconversion replaces long-range interactions that stabilize the chemokine fold with an entirely new set of tertiary and quaternary contacts. The chemokine-like Ltn conformation is a functional XCR1 agonist, but fails to bind heparin. In contrast, the alternative structure binds glycosaminoglycans with high affinity but fails to activate XCR1. Because each structural species displays only one of the two functional properties essential for activity in vivo, the conformational equilibrium is likely to be essential for the biological activity of lymphotactin. These results demonstrate that the functional repertoire and regulation of a single naturally occurring amino acid sequence can be expanded by access to a set of highly dissimilar native-state structures.
Interconversion between two unrelated protein folds in the lymphotactin native state.,Tuinstra RL, Peterson FC, Kutlesa S, Elgin ES, Kron MA, Volkman BF Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5057-62. Epub 2008 Mar 25. PMID:18364395[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Tuinstra RL, Peterson FC, Kutlesa S, Elgin ES, Kron MA, Volkman BF. Interconversion between two unrelated protein folds in the lymphotactin native state. Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5057-62. Epub 2008 Mar 25. PMID:18364395
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