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| | ==Solution Structure of T4 Bacteriophage Helicase Uvsw.1== | | ==Solution Structure of T4 Bacteriophage Helicase Uvsw.1== |
| - | <StructureSection load='2jpn' size='340' side='right' caption='[[2jpn]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2jpn' size='340' side='right'caption='[[2jpn]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2jpn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JPN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JPN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2jpn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JPN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JPN FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">uvsW, dar ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">uvsW, dar ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jpn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jpn OCA], [http://pdbe.org/2jpn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jpn RCSB], [http://www.ebi.ac.uk/pdbsum/2jpn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jpn ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jpn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jpn OCA], [https://pdbe.org/2jpn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jpn RCSB], [https://www.ebi.ac.uk/pdbsum/2jpn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jpn ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/UVSW_BPT4 UVSW_BPT4]] DNA helicase that catalyzes branch migration and dissociation of RNA-DNA hybrids. Plays a critical role in a specific recombination pathway involving simple reciprocal exchange. UvsW protein is normally the key regulatory factor in the switch from early to late phage DNA replication. | + | [[https://www.uniprot.org/uniprot/UVSW_BPT4 UVSW_BPT4]] DNA helicase that catalyzes branch migration and dissociation of RNA-DNA hybrids. Plays a critical role in a specific recombination pathway involving simple reciprocal exchange. UvsW protein is normally the key regulatory factor in the switch from early to late phage DNA replication. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Helicase|Helicase]] | + | *[[Helicase 3D structures|Helicase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Bpt4]] | | [[Category: Bpt4]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Kriwacki, R W]] | | [[Category: Kriwacki, R W]] |
| | [[Category: Lee, T]] | | [[Category: Lee, T]] |
| Structural highlights
Function
[UVSW_BPT4] DNA helicase that catalyzes branch migration and dissociation of RNA-DNA hybrids. Plays a critical role in a specific recombination pathway involving simple reciprocal exchange. UvsW protein is normally the key regulatory factor in the switch from early to late phage DNA replication.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The uvsWXY system is implicated in the replication and repair of the bacteriophage T4 genome. Whereas the roles of the recombinase (UvsX) and the recombination mediator protein (UvsY) are known, the precise role of UvsW is unclear. Sequence analysis identifies UvsW as a member of the monomeric SF2 helicase superfamily that translocates nucleic acid substrates via the action of two RecA-like motor domains. Functional homologies to Escherichia coli RecG and biochemical analyses have shown that UvsW interacts with branched nucleic acid substrates, suggesting roles in recombination and the rescue of stalled replication forks. A sequencing error at the 3'-end of the uvsW gene has revealed a second, short open reading frame that encodes a protein of unknown function called UvsW.1. We have determined the crystal structure of UvsW to 2.7A and the NMR solution structure of UvsW.1. UvsW has a four-domain architecture with structural homology to the eukaryotic SF2 helicase, Rad54. A model of the UvsW-ssDNA complex identifies structural elements and conserved residues that may interact with nucleic acid substrates. The NMR solution structure of UvsW.1 reveals a dynamic four-helix bundle with homology to the structure-specific nucleic acid binding module of RecQ helicases.
Crystallographic and NMR analyses of UvsW and UvsW.1 from bacteriophage T4.,Kerr ID, Sivakolundu S, Li Z, Buchsbaum JC, Knox LA, Kriwacki R, White SW J Biol Chem. 2007 Nov 23;282(47):34392-400. Epub 2007 Sep 17. PMID:17878153[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kerr ID, Sivakolundu S, Li Z, Buchsbaum JC, Knox LA, Kriwacki R, White SW. Crystallographic and NMR analyses of UvsW and UvsW.1 from bacteriophage T4. J Biol Chem. 2007 Nov 23;282(47):34392-400. Epub 2007 Sep 17. PMID:17878153 doi:10.1074/jbc.M705900200
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