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| | ==Structure of the transcription regulator SvtR from the hyperthermophilic archaeal virus SIRV1== | | ==Structure of the transcription regulator SvtR from the hyperthermophilic archaeal virus SIRV1== |
| - | <StructureSection load='2kel' size='340' side='right' caption='[[2kel]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2kel' size='340' side='right'caption='[[2kel]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2kel]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeal_virus_sirv Archaeal virus sirv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KEL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2kel]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeal_virus_sirv Archaeal virus sirv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KEL FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">56B, gp08 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=157898 Archaeal virus SIRV])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">56B, gp08 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=157898 Archaeal virus SIRV])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kel OCA], [http://pdbe.org/2kel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kel RCSB], [http://www.ebi.ac.uk/pdbsum/2kel PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kel ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kel OCA], [https://pdbe.org/2kel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kel RCSB], [https://www.ebi.ac.uk/pdbsum/2kel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kel ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Archaeal virus sirv]] | | [[Category: Archaeal virus sirv]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Delepierre, M]] | | [[Category: Delepierre, M]] |
| | [[Category: Guijarro, J I]] | | [[Category: Guijarro, J I]] |
| Structural highlights
Publication Abstract from PubMed
We have characterized the structure and the function of the 6.6-kDa protein SvtR (formerly called gp08) from the rod-shaped virus SIRV1, which infects the hyperthermophilic archaeon Sulfolobus islandicus that thrives at 85 degrees C in hot acidic springs. The protein forms a dimer in solution. The NMR solution structure of the protein consists of a ribbon-helix-helix (RHH) fold between residues 13 and 56 and a disordered N-terminal region (residues 1-12). The structure is very similar to that of bacterial RHH proteins despite the low sequence similarity. We demonstrated that the protein binds DNA and uses its beta-sheet face for the interaction like bacterial RHH proteins. To detect all the binding sites on the 32.3-kb SIRV1 linear genome, we designed and performed a global genome-wide search of targets based on a simplified electrophoretic mobility shift assay. Four targets were recognized by the protein. The strongest binding was observed with the promoter of the gene coding for a virion structural protein. When assayed in a host reconstituted in vitro transcription system, the protein SvtR (Sulfolobus virus transcription regulator) repressed transcription from the latter promoter, as well as from the promoter of its own gene.
Structure, function, and targets of the transcriptional regulator SvtR from the hyperthermophilic archaeal virus SIRV1.,Guilliere F, Peixeiro N, Kessler A, Raynal B, Desnoues N, Keller J, Delepierre M, Prangishvili D, Sezonov G, Guijarro JI J Biol Chem. 2009 Aug 14;284(33):22222-37. Epub 2009 Jun 17. PMID:19535331[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Guilliere F, Peixeiro N, Kessler A, Raynal B, Desnoues N, Keller J, Delepierre M, Prangishvili D, Sezonov G, Guijarro JI. Structure, function, and targets of the transcriptional regulator SvtR from the hyperthermophilic archaeal virus SIRV1. J Biol Chem. 2009 Aug 14;284(33):22222-37. Epub 2009 Jun 17. PMID:19535331 doi:10.1074/jbc.M109.029850
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