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7dmb
From Proteopedia
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==Crystal structure of trans-methyltransferase CalH complex with SAH== | ==Crystal structure of trans-methyltransferase CalH complex with SAH== | ||
| - | <StructureSection load='7dmb' size='340' side='right'caption='[[7dmb]]' scene=''> | + | <StructureSection load='7dmb' size='340' side='right'caption='[[7dmb]], [[Resolution|resolution]] 2.10Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DMB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DMB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7dmb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_16819 Atcc 16819]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DMB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DMB FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dmb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dmb OCA], [https://pdbe.org/7dmb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dmb RCSB], [https://www.ebi.ac.uk/pdbsum/7dmb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dmb ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dmb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dmb OCA], [https://pdbe.org/7dmb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dmb RCSB], [https://www.ebi.ac.uk/pdbsum/7dmb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dmb ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Calbistrins are fungal polyketides consisting of the characteristic decalin and polyene moieties. Although the biosynthetic gene cluster of calbistrin A was recently identified, the pathway of calbistrin A biosynthesis has largely remained uninvestigated. Herein, we investigated the mechanism by which the backbone structures of calbistrins are formed, by heterologous and in vitro reconstitution of the biosynthesis and a structural biological study. Intriguingly, our analyses revealed that the decalin and polyene portions of calbistrins are synthesized by the single polyketide synthase (PKS) CalA, with the aid of the trans-acting enoylreductase CalK and the trans-acting methyltransferase CalH, respectively. We also determined that the esterification of the two polyketide parts is catalyzed by the acyltransferase CalD. Our study has uncovered a novel dual-functional PKS and thus broadened our understanding of how fungi synthesize diverse polyketide natural products. | ||
| + | |||
| + | One Polyketide Synthase, Two Distinct Products: Trans-Acting Enzyme-Controlled Product Divergence in Calbistrin Biosynthesis.,Abe I, Tao H, Mori T, Wei X, Matsuda Y Angew Chem Int Ed Engl. 2021 Jan 22. doi: 10.1002/anie.202016525. PMID:33480463<ref>PMID:33480463</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7dmb" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Atcc 16819]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Abe I]] | + | [[Category: Abe, I]] |
| - | [[Category: Mori T]] | + | [[Category: Mori, T]] |
| - | [[Category: Tao H]] | + | [[Category: Tao, H]] |
| + | [[Category: Calbistrin a biosynthesis]] | ||
| + | [[Category: Calh]] | ||
| + | [[Category: Methyltransferase]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 07:02, 14 April 2021
Crystal structure of trans-methyltransferase CalH complex with SAH
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