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1jch

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Revision as of 07:18, 14 April 2021

Crystal Structure of Colicin E3 in Complex with its Immunity Protein

Structural highlights

1jch is a 4 chain structure with sequence from Ecow3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	3eip, 1e44
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CEA3_ECOLX] Inactivates ribosomes by hydrolyzing 16S RNA in 30S ribosomes at a specific site. Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. [IMM3_ECOLX] This protein inhibits the 16S RNA hydrolyzing activity of colicin E3 by binding with high affinity to the C-terminal catalytic domain of E3. This protein is able to protect a cell, which harbors the plasmid ColE3 against colicin E3.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Colicins kill E. coli by a process that involves binding to a surface receptor, entering the cell, and, finally, intoxicating it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site. The crystal structure of colicin E3, reported here in a binary complex with its immunity protein (IP), reveals a Y-shaped molecule with the receptor binding domain forming a 100 A long stalk and the two globular heads of the translocation domain (T) and the catalytic domain (C) comprising the two arms. Active site residues are D510, H513, E517, and R545. IP is buried between T and C. Rather than blocking the active site, IP prevents access of the active site to the ribosome.

Crystal structure of colicin E3: implications for cell entry and ribosome inactivation.,Soelaiman S, Jakes K, Wu N, Li C, Shoham M Mol Cell. 2001 Nov;8(5):1053-62. PMID:11741540^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Soelaiman S, Jakes K, Wu N, Li C, Shoham M. Crystal structure of colicin E3: implications for cell entry and ribosome inactivation. Mol Cell. 2001 Nov;8(5):1053-62. PMID:11741540

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jch"

@@ Line 3: / Line 3: @@
 <StructureSection load='1jch' size='340' side='right'caption='[[1jch]], [[Resolution|resolution]] 3.02&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1jch]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecow3 Ecow3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JCH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JCH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1jch]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecow3 Ecow3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JCH FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3eip|3eip]], [[1e44|1e44]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3eip|3eip]], [[1e44|1e44]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jch OCA], [http://pdbe.org/1jch PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jch RCSB], [http://www.ebi.ac.uk/pdbsum/1jch PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jch ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jch OCA], [https://pdbe.org/1jch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jch RCSB], [https://www.ebi.ac.uk/pdbsum/1jch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jch ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CEA3_ECOLX CEA3_ECOLX]] Inactivates ribosomes by hydrolyzing 16S RNA in 30S ribosomes at a specific site.  Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. [[http://www.uniprot.org/uniprot/IMM3_ECOLX IMM3_ECOLX]] This protein inhibits the 16S RNA hydrolyzing activity of colicin E3 by binding with high affinity to the C-terminal catalytic domain of E3. This protein is able to protect a cell, which harbors the plasmid ColE3 against colicin E3.
+[[https://www.uniprot.org/uniprot/CEA3_ECOLX CEA3_ECOLX]] Inactivates ribosomes by hydrolyzing 16S RNA in 30S ribosomes at a specific site.  Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. [[https://www.uniprot.org/uniprot/IMM3_ECOLX IMM3_ECOLX]] This protein inhibits the 16S RNA hydrolyzing activity of colicin E3 by binding with high affinity to the C-terminal catalytic domain of E3. This protein is able to protect a cell, which harbors the plasmid ColE3 against colicin E3.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 33: / Line 33: @@
 *[[Colicin 3D structures|Colicin 3D structures]]
 *[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
-*[[Im3|Im3]]
 == References ==
 <references/>

1jch

From Proteopedia

Revision as of 07:18, 14 April 2021

Crystal Structure of Colicin E3 in Complex with its Immunity Protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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