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| | ==Hyaloperonospora arabidopsidis Effector Protein ATR13== | | ==Hyaloperonospora arabidopsidis Effector Protein ATR13== |
| - | <StructureSection load='2lai' size='340' side='right' caption='[[2lai]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2lai' size='340' side='right'caption='[[2lai]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2lai]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hyaloperonospora_parasitica Hyaloperonospora parasitica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LAI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LAI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2lai]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hyaloperonospora_parasitica Hyaloperonospora parasitica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LAI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LAI FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Atr13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=123356 Hyaloperonospora parasitica])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Atr13 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=123356 Hyaloperonospora parasitica])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lai OCA], [http://pdbe.org/2lai PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lai RCSB], [http://www.ebi.ac.uk/pdbsum/2lai PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lai ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lai OCA], [https://pdbe.org/2lai PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lai RCSB], [https://www.ebi.ac.uk/pdbsum/2lai PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lai ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </div> | | </div> |
| | <div class="pdbe-citations 2lai" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 2lai" style="background-color:#fffaf0;"></div> |
| - | | |
| - | ==See Also== | |
| - | *[[Avirulence protein|Avirulence protein]] | |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Hyaloperonospora parasitica]] | | [[Category: Hyaloperonospora parasitica]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Leonelli, L]] | | [[Category: Leonelli, L]] |
| | [[Category: Pelton, J G]] | | [[Category: Pelton, J G]] |
| Structural highlights
Publication Abstract from PubMed
The oomycete Hyaloperonospora arabidopsidis (Hpa) is the causal agent of downy mildew on the model plant Arabidopsis thaliana and has been adapted as a model system to investigate pathogen virulence strategies and plant disease resistance mechanisms. Recognition of Hpa infection occurs when plant resistance proteins (R-genes) detect the presence or activity of pathogen-derived protein effectors delivered to the plant host. This study examines the Hpa effector ATR13 Emco5 and its recognition by RPP13-Nd, the cognate R-gene that triggers programmed cell death (HR) in the presence of recognized ATR13 variants. Herein, we use NMR to solve the backbone structure of ATR13 Emco5, revealing both a helical domain and a disordered internal loop. Additionally, we use site-directed and random mutagenesis to identify several amino acid residues involved in the recognition response conferred by RPP13-Nd. Using our structure as a scaffold, we map these residues to one of two surface-exposed patches of residues under diversifying selection. Exploring possible roles of the disordered region within the ATR13 structure, we perform domain swapping experiments and identify a peptide sequence involved in nucleolar localization. We conclude that ATR13 is a highly dynamic protein with no clear structural homologues that contains two surface-exposed patches of polymorphism, only one of which is involved in RPP13-Nd recognition specificity.
Structural Elucidation and Functional Characterization of the Hyaloperonospora arabidopsidis Effector Protein ATR13.,Leonelli L, Pelton J, Schoeffler A, Dahlbeck D, Berger J, Wemmer DE, Staskawicz B PLoS Pathog. 2011 Dec;7(12):e1002428. Epub 2011 Dec 15. PMID:22194684[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Leonelli L, Pelton J, Schoeffler A, Dahlbeck D, Berger J, Wemmer DE, Staskawicz B. Structural Elucidation and Functional Characterization of the Hyaloperonospora arabidopsidis Effector Protein ATR13. PLoS Pathog. 2011 Dec;7(12):e1002428. Epub 2011 Dec 15. PMID:22194684 doi:10.1371/journal.ppat.1002428
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