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Function of your protein

The protein in the article is Aldehyde dehydrogenase (AldC) and It has many functions. It helps with detoxification of aldehydes which are highly reactive compounds that are generated through cellular metabolism. It is also involved in biochemical functions from ethanol metabolism through oxidations, polyamine metabolism, and protective cellular response to stresses. The organism that is affection the protein is called Pseudomonas syringae (PtoDC3000) which is a mutation occurring in tomatoes.

Biological relevance and broader implications

The plant pathogen Pseudomonas syringae strain PtoDC3000 uses an indole-3-acetaldehyde dehydrogenase to synthesize the phytohormone indole-3-acetic acid to elude host responses. To suppress host defenses and promote diseases development, Pseudomonas syringae produces a variety of virulence factors, including phytohormones or chemical mimics of hormones, to manipulate hormone signaling in its host plants. P. syringae and many other plant-associated microbial pathogens can synthesize the major auxin indole-3-acetic acid (IAA), whose production is implicated in pathogen virulence. In this case the plant pathogen is affecting tomatoes, causing a mutation in them where the tomatoes dont have any defensed to fight off this plant pathogen.

As mentioned in the article, there is a variety of pseudomonas species that have evolved to grow under unfavorable environmental conditions like for example sever nutrient limitations, extreme temperatures, high salinity, low oxygen or water availability. P. syringae, a species that includes many plant pathogenic strains, developed

diverse bacterial virulence mechanisms to survive in the adverse environmental conditions of the phyllosphere.

This can be relevant to certain famers growing crops in different environments or climates. Pseudomonas syringae is a pathogen that can attack a wide variety of woody plant, it especially attacks them when the plants have been damaged by frost or an injury. Knowing this information farmers can be prepared so their crops are not affected by this pathogen. They can use a certain type of spray/chemical to protect them from it.

Important amino acids

4 Catalytic Residues () (blue color)

Asn 159, Glu 257, Gly 288, Cys 291(Cys 291 mutates to Ala 291)

(binding site, )

Ile 155, Asn 159, Lys 182, Gly 219, Ile 233-Ser 236, Ala 239, Leu 242, Glu 257, Leu 258, Gly 259, Cys 291, Glu 391, Phe 393

Trp 160, Tyr 163, Trp 450, Phe 456, Tyr 458, Met 114, Leu 118

In the article, there were other substrates mentioned that were detected in AldC by using spectrophotometric assay. Substrates that were identified were aliphatic aldehydes of 5–9-carbon length, like hydrocinnamaldehyde and 4-pyridinecarboxyaldehyde. As substrates, octanal had the highest specific activity that function properly for AldC. The article also mentions that short 2–4-carbon aldehydes, branched aliphatic aldehydes, and largeraromatic aldehydes are poor substrates for AldC.

Structural highlights

Secondary structure has feature and domains, the N-terminus of the protein contains a central Beta sheet that is surrounded by alpha helices to form the NAD(H)-binding site. Around the C-Terminus end of the protein it consists a mix of alpha/beta domains, which include a catalytic cysteine residue and forms the aldehyde -binding site. The article mentions as well what connects the N and C terminal domains of the protein AldC is by an interdomain linker region.

Other important features

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