6zt4
From Proteopedia
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==Pentapeptide repeat protein MfpA from Mycobacterium smegmatis== | ==Pentapeptide repeat protein MfpA from Mycobacterium smegmatis== | ||
| - | <StructureSection load='6zt4' size='340' side='right'caption='[[6zt4]]' scene=''> | + | <StructureSection load='6zt4' size='340' side='right'caption='[[6zt4]], [[Resolution|resolution]] 1.77Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZT4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZT4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6zt4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZT4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZT4 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zt4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zt4 OCA], [https://pdbe.org/6zt4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zt4 RCSB], [https://www.ebi.ac.uk/pdbsum/6zt4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zt4 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mfpA, MSMEG_1641, MSMEI_1602 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zt4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zt4 OCA], [https://pdbe.org/6zt4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zt4 RCSB], [https://www.ebi.ac.uk/pdbsum/6zt4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zt4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA gyrase, a type II topoisomerase, introduces negative supercoils into DNA using ATP hydrolysis. The highly effective gyrase-targeted drugs, fluoroquinolones (FQs), interrupt gyrase by stabilizing a DNA-cleavage complex, a transient intermediate in the supercoiling cycle, leading to double-stranded DNA breaks. MfpA, a pentapeptide-repeat protein in mycobacteria, protects gyrase from FQs, but its molecular mechanism remains unknown. Here, we show that Mycobacterium smegmatis MfpA (MsMfpA) inhibits negative supercoiling by M. smegmatis gyrase (Msgyrase) in the absence of FQs, while in their presence, MsMfpA decreases FQ-induced DNA cleavage, protecting the enzyme from these drugs. MsMfpA stimulates the ATPase activity of Msgyrase by directly interacting with the ATPase domain (MsGyrB47), which was confirmed through X-ray crystallography of the MsMfpA-MsGyrB47 complex, and mutational analysis, demonstrating that MsMfpA mimics a T (transported) DNA segment. These data reveal the molecular mechanism whereby MfpA modulates the activity of gyrase and may provide a general molecular basis for the action of other pentapeptide-repeat proteins. | ||
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| + | The pentapeptide-repeat protein, MfpA, interacts with mycobacterial DNA gyrase as a DNA T-segment mimic.,Feng L, Mundy JEA, Stevenson CEM, Mitchenall LA, Lawson DM, Mi K, Maxwell A Proc Natl Acad Sci U S A. 2021 Mar 16;118(11). pii: 2016705118. doi:, 10.1073/pnas.2016705118. PMID:33836580<ref>PMID:33836580</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6zt4" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Feng L]] | + | [[Category: Mycs2]] |
| - | [[Category: Lawson | + | [[Category: Feng, L]] |
| - | [[Category: Maxwell A]] | + | [[Category: Lawson, D M]] |
| - | [[Category: Mi K]] | + | [[Category: Maxwell, A]] |
| - | [[Category: Mitchenall | + | [[Category: Mi, K]] |
| - | [[Category: Mundy | + | [[Category: Mitchenall, L A]] |
| - | [[Category: Stevenson | + | [[Category: Mundy, J E.A]] |
| + | [[Category: Stevenson, C E.M]] | ||
| + | [[Category: Dna gyrase]] | ||
| + | [[Category: Dna mimicry]] | ||
| + | [[Category: Fluoroquinolone resistance]] | ||
| + | [[Category: Pentapeptide repeat protein]] | ||
| + | [[Category: Protein binding]] | ||
| + | [[Category: Right-handed quadrilateral beta-helix]] | ||
Revision as of 08:30, 21 April 2021
Pentapeptide repeat protein MfpA from Mycobacterium smegmatis
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