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Sandbox GGC16
From Proteopedia
(Difference between revisions)
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==PU.1== | ==PU.1== | ||
<StructureSection load='1PUE' size='340' side='right' caption='Caption for this structure' scene=''> | <StructureSection load='1PUE' size='340' side='right' caption='Caption for this structure' scene=''> | ||
| - | PU.1 is a hematopoietic transcription factor. It specifically regulates transcription of genes specific to myeloid and lymphoid cells, ultimately leading to cellular differentiation. | + | PU.1 is a hematopoietic transcription factor. It specifically regulates transcription of genes specific to myeloid and lymphoid cells, ultimately leading to cellular differentiation. This transcription factor binds DNA with its helix-turn-helix motif. |
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===Binding Domain=== | ===Binding Domain=== | ||
<scene name='87/874948/Earbindingresiduesbest/1'>Residues at Binding Domain</scene> | <scene name='87/874948/Earbindingresiduesbest/1'>Residues at Binding Domain</scene> | ||
| - | Ets binding domain is conserved across the Ets family of proteins. | + | Ets binding domain is conserved across the Ets family of proteins which consist of about 35 proteins with similar functions. |
| - | Binding domain is positively charged. Residues most closely interacting with DNA include Arginine and Lysine. | + | Binding domain is positively charged. Residues most closely interacting with DNA include Arginine and Lysine. These are highly conserved- the two Arginines on the central helix within the major groove of the DNA and two Lysines on the outer turns. |
== Disease == | == Disease == | ||
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== PEST domain == | == PEST domain == | ||
| + | The PEST domain is adjacent to the binding domain. It is highly polar due to the abundance of proline, glutamate, serine, and threonine. Proteins with a PEST region typically have a short half-life of under 2 hours. It is hypothesized that the PEST region is a tag for fast degradation of the protein. | ||
== Structural highlights == | == Structural highlights == | ||
Revision as of 16:34, 24 April 2021
PU.1
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