6xhs
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Crystal structure of S. aureus TarI in complex with CTP (space group P1211)== | ==Crystal structure of S. aureus TarI in complex with CTP (space group P1211)== | ||
| - | <StructureSection load='6xhs' size='340' side='right'caption='[[6xhs]]' scene=''> | + | <StructureSection load='6xhs' size='340' side='right'caption='[[6xhs]], [[Resolution|resolution]] 2.90Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XHS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6xhs]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XHS FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xhs OCA], [https://pdbe.org/6xhs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xhs RCSB], [https://www.ebi.ac.uk/pdbsum/6xhs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xhs ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CTP:CYTIDINE-5-TRIPHOSPHATE'>CTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> |
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/D-ribitol-5-phosphate_cytidylyltransferase D-ribitol-5-phosphate cytidylyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.40 2.7.7.40] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xhs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xhs OCA], [https://pdbe.org/6xhs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xhs RCSB], [https://www.ebi.ac.uk/pdbsum/6xhs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xhs ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/TARI1_STAA8 TARI1_STAA8]] Catalyzes the transfer of the cytidylyl group of CTP to D-ribitol 5-phosphate.[HAMAP-Rule:MF_02068]<ref>PMID:15362865</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The cell wall of many pathogenic Gram-positive bacteria contains ribitol-phosphate wall teichoic acid (WTA), a polymer that is linked to virulence and regulation of essential physiological processes including cell division. CDP-ribitol, the activated precursor for ribitol-phosphate polymerization, is synthesized by a cytidylyltransferase and reductase pair known as TarI and TarJ, respectively. In this study, we present crystal structures of Staphylococcus aureus TarI and TarJ in their apo forms and in complex with substrates and products. The TarI structures illustrate the mechanism of CDP-ribitol synthesis from CTP and ribitol-phosphate and reveal structural changes required for substrate binding and catalysis. Insights into the upstream step of ribulose-phosphate reduction to ribitol-phosphate is provided by the structures of TarJ. Furthermore, we propose a general topology of the enzymes in a heterotetrameric form built using restraints from crosslinking mass spectrometry analysis. Together, our data present molecular details of CDP-ribitol production that may aid in the design of inhibitors against WTA biosynthesis. | ||
| + | |||
| + | Crystallographic analysis of TarI and TarJ, a cytidylyltransferase and reductase pair for CDP-ribitol synthesis in Staphylococcus aureus wall teichoic acid biogenesis.,Li FKK, Gale RT, Petrotchenko EV, Borchers CH, Brown ED, Strynadka NCJ J Struct Biol. 2021 Apr 2:107733. doi: 10.1016/j.jsb.2021.107733. PMID:33819634<ref>PMID:33819634</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6xhs" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: D-ribitol-5-phosphate cytidylyltransferase]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Li | + | [[Category: Li, F K.K]] |
| - | [[Category: Strynadka | + | [[Category: Strynadka, N C.J]] |
| + | [[Category: Cytidylyltransferase]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 05:28, 28 April 2021
Crystal structure of S. aureus TarI in complex with CTP (space group P1211)
| |||||||||||
