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1m9g

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Revision as of 05:58, 28 April 2021

Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI

Structural highlights

1m9g is a 1 chain structure with sequence from Diocu. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MONB_DIOCU] Taste-modifying protein; intensely sweet-tasting protein.

Publication Abstract from PubMed

The mechanism by which sweet proteins elicit a response on the T1R2-T1R3 sweet taste receptor is still mostly unknown but has been so far related to the presence of "sweet fingers" on the protein surface able to interact with the same mechanism as that of low molecular mass sweeteners. In the search for the identification of sweet fingers, we have solved the solution structure of G16A MNEI, a structural mutant that shows a reduction of one order of magnitude in sweetness with respect to its parent protein, MNEI, a single-chain monellin. Comparison of the structures of wild-type monellin and its G16A mutant shows that the mutation does not affect the structure of potential glucophores but produces a distortion of the surface owing to the partial relative displacement of elements of secondary structure. These results show conclusively that sweet proteins do not possess a sweet finger and strongly support the hypothesis that the mechanism of interaction of sweet-tasting proteins with the recently identified T1R2-T1R3 GPC receptor is different from that of low molecular mass sweeteners.

The mechanism of interaction of sweet proteins with the T1R2-T1R3 receptor: evidence from the solution structure of G16A-MNEI.,Spadaccini R, Trabucco F, Saviano G, Picone D, Crescenzi O, Tancredi T, Temussi PA J Mol Biol. 2003 May 2;328(3):683-92. PMID:12706725^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Spadaccini R, Trabucco F, Saviano G, Picone D, Crescenzi O, Tancredi T, Temussi PA. The mechanism of interaction of sweet proteins with the T1R2-T1R3 receptor: evidence from the solution structure of G16A-MNEI. J Mol Biol. 2003 May 2;328(3):683-92. PMID:12706725

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1m9g"

@@ Line 3: / Line 3: @@
 <StructureSection load='1m9g' size='340' side='right'caption='[[1m9g]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M9G FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1m9g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Diocu Diocu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M9G FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9g OCA], [http://pdbe.org/1m9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1m9g RCSB], [http://www.ebi.ac.uk/pdbsum/1m9g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1m9g ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m9g OCA], [https://pdbe.org/1m9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m9g RCSB], [https://www.ebi.ac.uk/pdbsum/1m9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m9g ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU]] Taste-modifying protein; intensely sweet-tasting protein.
+[[https://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU]] Taste-modifying protein; intensely sweet-tasting protein.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

1m9g

From Proteopedia

Revision as of 05:58, 28 April 2021

Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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