Sandbox GGC12

Human serum albumin or HSA is one of the major types of proteins that are present in the plasma composition. It is such abundant that its concentration on a common blood sample is 5 grams per 100 milliliters. Due to its high concentration in plasma as well as its physiological and pharmaceutical features, it has been subjected to several studies to determine its 3D structure, function, domains, important binding sites, and diseases. The primary structure of HSA describes a single polypeptide with 585 amino acids with the characteristics of having 17 pairs of disulfide bridges, one free cysteine ^[1]. It has been discovered that there are highly conserved sequences between bovine, human and rat albumins such as Trp-212, 143-155 and 244-263 sequence ^[2]. It is composed of three domains at positions 19-210, 211-403, and 404-601 with two subdomains each (Ia&b, IIa&b, and IIIa&b). The subcellular location of these protein is the extracellular region on the outside of the cell membrane or secreted. There are 11 metal binding sites, including 1 copper site, 7 calcium sites, 3 zinc sites, additionally, 1 binding site for bilirubin and 1 site for aspirin-acetylated lysine ^[3].

Function

HSA functions as the essential protein for the circulatory system, however, it has been discovered that possesses a high affinity an extensive range of amino acids, ligands, fatty acids, metals like copper and zinc, and metabolites such as bilirubin and drugs ingredients ^[4].Its fundamental role is to transport these varied types of solutes through the bloodstream to specific organs, control the level of pH and osmotic pressure of the plasma, and interaction with the cell membrane exchange of molecules at binding sites ^[5]. It is considered to be the vital carrier of zinc in the plasma as well as the calcium and magnesium. In terms of affinity, the order from higher to lower affinity follows this pattern: zinc, calcium and magnesium ^[6]. Pharmaceutically, it has been used to target certain organs with some drugs by HSA being the carrier. By performing experiments on E.coli, it was discovered that it inhibits enterobactin-mediated iron absorption from ferric transferrin due to its binding to the bacterial siderophore limiting the uptake of iron, and consequently, blocking the growth of E.coli ^[7].

Disease

HSA is involved in two important diseases, Hyperthyroxinemia, familial dysalbuminemic (FDAH) and Analbuminemia (ANALBA).

First, FDAH is a condition based on the genetic composition of the individual. It is caused by a mutation in the ALB gene, which corresponds to an increased affinity of the protein HSA for thyroxine. In detail, this autosomal dominant genetic disorder is characterized by the mutation of HSA causing the assembly of thyroxine in a higher proportion. There are two positions with three natural variants identified on the HSA that mutate to cause this disorder. The first position is at 90 amino acid, where leucine is replaced by a proline. The second position at 242 amino acid, where arginine is substituted by histidine or proline. This disorder could lead to confusion and several misdiagnoses of hyperthyroidism because it is difficult to detect it due to the normal TSH and free thyroxine levels but with an elevated total of thyroxine ^[8].

Second, ANALBA is an uncommon autosomal recessive genetic mutation characterized by the identification of very low levels of HSA circulating in the bloodstream ^[9]. The affected individuals have symptoms corresponding to mild edema, hypotension, fatigue, and lower body lipodystrophy in females. The mutagenesis is located in the position 91 where histidine is replaced by alanine impairing metal binding ^[10]. The complications of this disorder could lead to early atherosclerosis and heart problems.

Relevance

The relevance of this protein, HSA, relies on the its capacity to transports molecules through out the bloodstream. It helps move substances such as metals, amino acids, drugs, fatty acids to every part of the body because it is a protein present in the blood that flows around the body. It is a significant protein because it allows us to use it pharmaceutically to target certain organs with specific drugs. Additionally, it is essential for the control of the levels of pH and the osmotic pressure in the plasma. And finally, it is a protein that allows the cell membrane interact with the outer space of the cell by the help of HSA in the targeting of binding sites.

Structural highlights

The structural highlights of this protein are four: the three domains that compose the protein, the binding site in domain II for salicylates, sulfonamides and several drug ingredients, the bilirubin binding site at position 264, and the free cysteine in the structure of the protein ^[11].

This is a sample scene created with SAT to by Group, and another to make of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.