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7lhl
From Proteopedia
(Difference between revisions)
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==cryo-EM structure of Mycobacterium smegmatis Lhr helicase C-terminal domain== | ==cryo-EM structure of Mycobacterium smegmatis Lhr helicase C-terminal domain== | ||
| - | <StructureSection load='7lhl' size='340' side='right'caption='[[7lhl]]' scene=''> | + | <StructureSection load='7lhl' size='340' side='right'caption='[[7lhl]], [[Resolution|resolution]] 3.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LHL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7lhl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_smegmatis"_trevisan_1889 "bacillus smegmatis" trevisan 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LHL FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lhl OCA], [https://pdbe.org/7lhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lhl RCSB], [https://www.ebi.ac.uk/pdbsum/7lhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lhl ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lhr, ERS451418_01790 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1772 "Bacillus smegmatis" Trevisan 1889])</td></tr> |
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lhl OCA], [https://pdbe.org/7lhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lhl RCSB], [https://www.ebi.ac.uk/pdbsum/7lhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lhl ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mycobacterium smegmatis Lhr (MsmLhr; 1507-aa) is the founder of a novel clade of bacterial helicases. MsmLhr consists of an N-terminal helicase domain (aa 1-856) with a distinctive tertiary structure (Lhr-Core) and a C-terminal domain (Lhr-CTD) of unknown structure. Here, we report that Escherichia coli Lhr (EcoLhr; 1538-aa) is an ATPase, translocase and ATP-dependent helicase. Like MsmLhr, EcoLhr translocates 3' to 5' on ssDNA and unwinds secondary structures en route, with RNA:DNA hybrid being preferred versus DNA:DNA duplex. The ATPase and translocase activities of EcoLhr inhere to its 877-aa Core domain. Full-length EcoLhr and MsmLhr have homo-oligomeric quaternary structures in solution, whereas their respective Core domains are monomers. The MsmLhr CTD per se is a homo-oligomer in solution. We employed cryo-EM to solve the structure of the CTD of full-length MsmLhr. The CTD protomer is composed of a series of five winged-helix (WH) modules and a beta-barrel module. The CTD adopts a unique homo-tetrameric quaternary structure. A Lhr-CTD subdomain, comprising three tandem WH modules and the beta-barrel, is structurally homologous to AlkZ, a bacterial DNA glycosylase that recognizes and excises inter-strand DNA crosslinks. This homology is noteworthy given that Lhr is induced in mycobacteria exposed to the inter-strand crosslinker mitomycin C. | ||
| + | |||
| + | Oligomeric quaternary structure of Escherichia coli and Mycobacterium smegmatis Lhr helicases is nucleated by a novel C-terminal domain composed of five winged-helix modules.,Warren GM, Wang J, Patel DJ, Shuman S Nucleic Acids Res. 2021 Mar 21. pii: 6179347. doi: 10.1093/nar/gkab145. PMID:33744958<ref>PMID:33744958</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7lhl" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bacillus smegmatis trevisan 1889]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Patel | + | [[Category: RNA helicase]] |
| - | [[Category: Shuman S]] | + | [[Category: Patel, D J]] |
| - | [[Category: Wang J]] | + | [[Category: Shuman, S]] |
| - | [[Category: Warren | + | [[Category: Wang, J]] |
| + | [[Category: Warren, G M]] | ||
| + | [[Category: Helicase]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Winged helix]] | ||
Revision as of 09:14, 5 May 2021
cryo-EM structure of Mycobacterium smegmatis Lhr helicase C-terminal domain
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