1ed0
From Proteopedia
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'''NMR structural determination of viscotoxin A3 from Viscum album L.''' | '''NMR structural determination of viscotoxin A3 from Viscum album L.''' | ||
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[[Category: Ugolini, R.]] | [[Category: Ugolini, R.]] | ||
[[Category: Urech, K.]] | [[Category: Urech, K.]] | ||
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| - | [[Category: | + | [[Category: Helix-turn-helix]] |
| - | [[Category: | + | [[Category: Thionin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:56:58 2008'' | |
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Revision as of 11:56, 2 May 2008
NMR structural determination of viscotoxin A3 from Viscum album L.
Overview
The high-resolution three-dimensional structure of the plant toxin viscotoxin A3, from Viscum album L., has been determined in solution by (1)H NMR spectroscopy at pH 3.6 and 12 degrees C (the structure has been deposited in the Protein Data Bank under the id. code 1ED0). Experimentally derived restraints including 734 interproton distances from nuclear Overhauser effect measurements, 22 hydrogen bonds, 32 φ angle restraints from J coupling measurements, together with three disulphide bridge constraints were used as input in restrained molecular dynamics, followed by minimization, using DYANA and Discover. Backbone and heavy atom root-mean-square deviations were 0.47+/-0.11 A (1 A=10(-10) m) and 0.85+/-0.13 A respectively. Viscotoxin A3 consists of two alpha-helices connected by a turn and a short stretch of antiparallel beta-sheet. This fold is similar to that found in other thionins, such as crambin, hordothionin-alpha and -beta, phoratoxin A and purothionin-alpha and -beta. The difference in the observed biological activity for thionins of known structure is discussed in terms of the differences in the calculated surface potential distribution, playing an important role in their function through disruption of cell membranes. In addition, the possible role in DNA binding of the helix-turn-helix motif of viscotoxin A3 is discussed.
About this Structure
1ED0 is a Single protein structure of sequence from Viscum album. Full crystallographic information is available from OCA.
Reference
NMR structural determination of viscotoxin A3 from Viscum album L., Romagnoli S, Ugolini R, Fogolari F, Schaller G, Urech K, Giannattasio M, Ragona L, Molinari H, Biochem J. 2000 Sep 1;350 Pt 2:569-77. PMID:10947973 Page seeded by OCA on Fri May 2 14:56:58 2008
