1ee6

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[[Image:1ee6.gif|left|200px]]
[[Image:1ee6.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1ee6 |SIZE=350|CAPTION= <scene name='initialview01'>1ee6</scene>, resolution 2.3&Aring;
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The line below this paragraph, containing "STRUCTURE_1ee6", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pectate_lyase Pectate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.2 4.2.2.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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{{STRUCTURE_1ee6| PDB=1ee6 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ee6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ee6 OCA], [http://www.ebi.ac.uk/pdbsum/1ee6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ee6 RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF PECTATE LYASE FROM BACILLUS SP. STRAIN KSM-P15.'''
'''CRYSTAL STRUCTURE OF PECTATE LYASE FROM BACILLUS SP. STRAIN KSM-P15.'''
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[[Category: Suzuki, A.]]
[[Category: Suzuki, A.]]
[[Category: Yamane, T.]]
[[Category: Yamane, T.]]
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[[Category: high-alkaline]]
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[[Category: High-alkaline]]
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[[Category: low-molecular-weight]]
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[[Category: Low-molecular-weight]]
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[[Category: parallel beta-helix]]
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[[Category: Parallel beta-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:59:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:00:47 2008''
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Revision as of 11:59, 2 May 2008

Image:1ee6.gif

Template:STRUCTURE 1ee6

CRYSTAL STRUCTURE OF PECTATE LYASE FROM BACILLUS SP. STRAIN KSM-P15.


Overview

The crystal structure of a highly alkaline low molecular weight pectate lyase (Pel-15) was determined at 1.5 A resolution by the multiple isomorphous replacement (MIR) method. This is the first pectate lyase structure from polysaccharide lyase family 3. The overall structure is a simple eight-turn right-handed parallel beta-helix domain with one long loop protruding from one side of the beta-helix. The low molecular weight of Pel-15 derives from the lack of N- and C-terminal extensions that are found in many beta-helix proteins. Although the structure has one calcium ion at pH 6.7, raising the pH to 9.5 results in the binding of an additional calcium ion. The common calcium ion found in both the pH 6.5 and 9.5 structures seems to stabilize both the beta-helix structure and the long protruding loop. The additional calcium ion found in the pH 9.5 structure alone may neutralize the acidic substrate. The region around the additional calcium ion is thought to bind to the substrate, as this region is rich in charged amino-acid residues which are required in catalysis.

About this Structure

1EE6 is a Single protein structure of sequence from Bacillus sp.. Full crystallographic information is available from OCA.

Reference

The first structure of pectate lyase belonging to polysaccharide lyase family 3., Akita M, Suzuki A, Kobayashi T, Ito S, Yamane T, Acta Crystallogr D Biol Crystallogr. 2001 Dec;57(Pt 12):1786-92. Epub 2001, Nov 21. PMID:11717490 Page seeded by OCA on Fri May 2 14:59:14 2008

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