1ee8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1ee8.gif|left|200px]]
[[Image:1ee8.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1ee8 |SIZE=350|CAPTION= <scene name='initialview01'>1ee8</scene>, resolution 1.9&Aring;
+
The line below this paragraph, containing "STRUCTURE_1ee8", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1ee8| PDB=1ee8 | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ee8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ee8 OCA], [http://www.ebi.ac.uk/pdbsum/1ee8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ee8 RCSB]</span>
+
-
}}
+
'''CRYSTAL STRUCTURE OF MUTM (FPG) PROTEIN FROM THERMUS THERMOPHILUS HB8'''
'''CRYSTAL STRUCTURE OF MUTM (FPG) PROTEIN FROM THERMUS THERMOPHILUS HB8'''
Line 34: Line 31:
[[Category: Sugahara, M.]]
[[Category: Sugahara, M.]]
[[Category: Yamamoto, M.]]
[[Category: Yamamoto, M.]]
-
[[Category: beta sandwich]]
+
[[Category: Beta sandwich]]
-
[[Category: helix two-turns helix]]
+
[[Category: Helix two-turns helix]]
-
[[Category: riken structural genomics/proteomics initiative]]
+
[[Category: Riken structural genomics/proteomics initiative]]
-
[[Category: rsgi]]
+
[[Category: Rsgi]]
-
[[Category: structural genomic]]
+
[[Category: Structural genomic]]
-
[[Category: zinc finger]]
+
[[Category: Zinc finger]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:59:20 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:00:54 2008''
+

Revision as of 11:59, 2 May 2008

Image:1ee8.gif

Template:STRUCTURE 1ee8

CRYSTAL STRUCTURE OF MUTM (FPG) PROTEIN FROM THERMUS THERMOPHILUS HB8


Overview

The MutM [formamidopyrimidine DNA glycosylase (Fpg)] protein is a trifunctional DNA base excision repair enzyme that removes a wide range of oxidatively damaged bases (N-glycosylase activity) and cleaves both the 3'- and 5'-phosphodiester bonds of the resulting apurinic/apyrimidinic site (AP lyase activity). The crystal structure of MutM from an extreme thermophile, Thermus thermophilus HB8, was determined at 1.9 A resolution with multiwavelength anomalous diffraction phasing using the intrinsic Zn(2+) ion of the zinc finger. MutM is composed of two distinct and novel domains connected by a flexible hinge. There is a large, electrostatically positive cleft lined by highly conserved residues between the domains. On the basis of the three-dimensional structure and taking account of previous biochemical experiments, we propose a DNA-binding mode and reaction mechanism for MutM. The locations of the putative catalytic residues and the two DNA-binding motifs (the zinc finger and the helix-two-turns-helix motifs) suggest that the oxidized base is flipped out from double-stranded DNA in the binding mode and excised by a catalytic mechanism similar to that of bifunctional base excision repair enzymes.

About this Structure

1EE8 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Crystal structure of a repair enzyme of oxidatively damaged DNA, MutM (Fpg), from an extreme thermophile, Thermus thermophilus HB8., Sugahara M, Mikawa T, Kumasaka T, Yamamoto M, Kato R, Fukuyama K, Inoue Y, Kuramitsu S, EMBO J. 2000 Aug 1;19(15):3857-69. PMID:10921868 Page seeded by OCA on Fri May 2 14:59:20 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ee8"
Personal tools