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2wfz

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Revision as of 09:25, 12 May 2021

NON-AGED CONJUGATE OF TORPEDO CALIFORNICA ACETYLCHOLINESTERASE WITH SOMAN

Structural highlights

2wfz is a 1 chain structure with sequence from Torpedo californica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , , , ,
Related:	1zgb, 1amn, 1qti, 1e66, 2vq6, 2ack, 2j3d, 1qii, 2ckm, 1dx6, 1qij, 1qie, 1acl, 1w4l, 1odc, 2cmf, 1gqs, 2j3q, 1e3q, 2j4f, 2dfp, 1qik, 2c5f, 1ea5, 1eea, 1qif, 2vjc, 2vjb, 1qig, 1qid, 1zgc, 2vjd, 1jjb, 1ut6, 2vt6, 2w9i, 2vt7, 2cek, 1qim, 1gpk, 1jga, 3ace, 1oce, 1w6r, 1som, 1vxo, 2w6c, 2vja, 1cfj, 2v96, 1ax9, 1u65, 1w76, 1h22, 1eve, 2c4h, 2ace, 2va9, 1gqr, 1vxr, 4ace, 2c58, 1hbj, 1w75, 1vot, 2c5g, 2v98, 1jgb, 1gpn, 1qih, 1h23, 1acj, 1fss, 2v97, 2wg2, 2wg1, 2wg0
Activity:	Acetylcholinesterase, with EC number 3.1.1.7
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ACES_TORCA] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Organophosphate compounds (OP) are potent inhibitors of acetylcholinesterases (AChEs) and can cause lethal poisoning in humans. Inhibition of AChEs by the OP soman involves phosphonylation of the catalytic serine, and subsequent dealkylation produces a form known as the "aged" enzyme. The nonaged form can be reactivated to a certain extent by nucleophiles, such as pralidoxime (2-PAM), whereas aged forms of OP-inhibited AChEs are totally resistant to reactivation. Here, we solved the X-ray crystal structures of AChE from Torpedo californica (TcAChE) conjugated with soman before and after aging. The absolute configuration of the soman stereoisomer adduct in the nonaged conjugate is P(S)C(R). A structural reorientation of the catalytic His440 side chain was observed during the aging process. Furthermore, the crystal structure of the ternary complex of the aged conjugate with 2-PAM revealed that the orientation of the oxime function does not permit nucleophilic attack on the phosphorus atom, thus providing a plausible explanation for its failure to reactivate the aged soman/AChE conjugate. Together, these three crystal structures provide an experimental basis for the design of new reactivators.

Crystallographic Snapshots of Nonaged and Aged Conjugates of Soman with Acetylcholinesterase, and of a Ternary Complex of the Aged Conjugate with Pralidoxime (dagger).,Sanson B, Nachon F, Colletier JP, Froment MT, Toker L, Greenblatt HM, Sussman JL, Ashani Y, Masson P, Silman I, Weik M J Med Chem. 2009 Jul 30. PMID:19642642^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sanson B, Nachon F, Colletier JP, Froment MT, Toker L, Greenblatt HM, Sussman JL, Ashani Y, Masson P, Silman I, Weik M. Crystallographic Snapshots of Nonaged and Aged Conjugates of Soman with Acetylcholinesterase, and of a Ternary Complex of the Aged Conjugate with Pralidoxime (dagger). J Med Chem. 2009 Jul 30. PMID:19642642 doi:10.1021/jm900433t

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wfz"

@@ Line 3: / Line 3: @@
 <StructureSection load='2wfz' size='340' side='right'caption='[[2wfz]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wfz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WFZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wfz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WFZ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GD8:(1R)-1,2,2-TRIMETHYLPROPYL+(S)-METHYLPHOSPHINATE'>GD8</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GD8:(1R)-1,2,2-TRIMETHYLPROPYL+(S)-METHYLPHOSPHINATE'>GD8</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zgb|1zgb]], [[1amn|1amn]], [[1qti|1qti]], [[1e66|1e66]], [[2vq6|2vq6]], [[2ack|2ack]], [[2j3d|2j3d]], [[1qii|1qii]], [[2ckm|2ckm]], [[1dx6|1dx6]], [[1qij|1qij]], [[1qie|1qie]], [[1acl|1acl]], [[1w4l|1w4l]], [[1odc|1odc]], [[2cmf|2cmf]], [[1gqs|1gqs]], [[2j3q|2j3q]], [[1e3q|1e3q]], [[2j4f|2j4f]], [[2dfp|2dfp]], [[1qik|1qik]], [[2c5f|2c5f]], [[1ea5|1ea5]], [[1eea|1eea]], [[1qif|1qif]], [[2vjc|2vjc]], [[2vjb|2vjb]], [[1qig|1qig]], [[1qid|1qid]], [[1zgc|1zgc]], [[2vjd|2vjd]], [[1jjb|1jjb]], [[1ut6|1ut6]], [[2vt6|2vt6]], [[2w9i|2w9i]], [[2vt7|2vt7]], [[2cek|2cek]], [[1qim|1qim]], [[1gpk|1gpk]], [[1jga|1jga]], [[3ace|3ace]], [[1oce|1oce]], [[1w6r|1w6r]], [[1som|1som]], [[1vxo|1vxo]], [[2w6c|2w6c]], [[2vja|2vja]], [[1cfj|1cfj]], [[2v96|2v96]], [[1ax9|1ax9]], [[1u65|1u65]], [[1w76|1w76]], [[1h22|1h22]], [[1eve|1eve]], [[2c4h|2c4h]], [[2ace|2ace]], [[2va9|2va9]], [[1gqr|1gqr]], [[1vxr|1vxr]], [[4ace|4ace]], [[2c58|2c58]], [[1hbj|1hbj]], [[1w75|1w75]], [[1vot|1vot]], [[2c5g|2c5g]], [[2v98|2v98]], [[1jgb|1jgb]], [[1gpn|1gpn]], [[1qih|1qih]], [[1h23|1h23]], [[1acj|1acj]], [[1fss|1fss]], [[2v97|2v97]], [[2wg2|2wg2]], [[2wg1|2wg1]], [[2wg0|2wg0]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1zgb|1zgb]], [[1amn|1amn]], [[1qti|1qti]], [[1e66|1e66]], [[2vq6|2vq6]], [[2ack|2ack]], [[2j3d|2j3d]], [[1qii|1qii]], [[2ckm|2ckm]], [[1dx6|1dx6]], [[1qij|1qij]], [[1qie|1qie]], [[1acl|1acl]], [[1w4l|1w4l]], [[1odc|1odc]], [[2cmf|2cmf]], [[1gqs|1gqs]], [[2j3q|2j3q]], [[1e3q|1e3q]], [[2j4f|2j4f]], [[2dfp|2dfp]], [[1qik|1qik]], [[2c5f|2c5f]], [[1ea5|1ea5]], [[1eea|1eea]], [[1qif|1qif]], [[2vjc|2vjc]], [[2vjb|2vjb]], [[1qig|1qig]], [[1qid|1qid]], [[1zgc|1zgc]], [[2vjd|2vjd]], [[1jjb|1jjb]], [[1ut6|1ut6]], [[2vt6|2vt6]], [[2w9i|2w9i]], [[2vt7|2vt7]], [[2cek|2cek]], [[1qim|1qim]], [[1gpk|1gpk]], [[1jga|1jga]], [[3ace|3ace]], [[1oce|1oce]], [[1w6r|1w6r]], [[1som|1som]], [[1vxo|1vxo]], [[2w6c|2w6c]], [[2vja|2vja]], [[1cfj|1cfj]], [[2v96|2v96]], [[1ax9|1ax9]], [[1u65|1u65]], [[1w76|1w76]], [[1h22|1h22]], [[1eve|1eve]], [[2c4h|2c4h]], [[2ace|2ace]], [[2va9|2va9]], [[1gqr|1gqr]], [[1vxr|1vxr]], [[4ace|4ace]], [[2c58|2c58]], [[1hbj|1hbj]], [[1w75|1w75]], [[1vot|1vot]], [[2c5g|2c5g]], [[2v98|2v98]], [[1jgb|1jgb]], [[1gpn|1gpn]], [[1qih|1qih]], [[1h23|1h23]], [[1acj|1acj]], [[1fss|1fss]], [[2v97|2v97]], [[2wg2|2wg2]], [[2wg1|2wg1]], [[2wg0|2wg0]]</div></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wfz OCA], [http://pdbe.org/2wfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wfz RCSB], [http://www.ebi.ac.uk/pdbsum/2wfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wfz ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wfz OCA], [https://pdbe.org/2wfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wfz RCSB], [https://www.ebi.ac.uk/pdbsum/2wfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wfz ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ACES_TORCA ACES_TORCA]] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.
+[[https://www.uniprot.org/uniprot/ACES_TORCA ACES_TORCA]] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 32: @@
 ==See Also==
-*[[AChE inhibitors and substrates|AChE inhibitors and substrates]]
 *[[Acetylcholinesterase|Acetylcholinesterase]]
 *[[Acetylcholinesterase 3D structures|Acetylcholinesterase 3D structures]]
-*[[Acetylcholinesterase inhibited by nerve agent soman|Acetylcholinesterase inhibited by nerve agent soman]]
-*[[Acetylcholinesterase with DFP|Acetylcholinesterase with DFP]]
 == References ==
 <references/>

2wfz

From Proteopedia

Revision as of 09:25, 12 May 2021

NON-AGED CONJUGATE OF TORPEDO CALIFORNICA ACETYLCHOLINESTERASE WITH SOMAN

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

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