1ef0
From Proteopedia
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'''CRYSTAL STRUCTURE OF PI-SCEI MINIPRECURSOR''' | '''CRYSTAL STRUCTURE OF PI-SCEI MINIPRECURSOR''' | ||
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==Reference== | ==Reference== | ||
Structural insights into the protein splicing mechanism of PI-SceI., Poland BW, Xu MQ, Quiocho FA, J Biol Chem. 2000 Jun 2;275(22):16408-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10828056 10828056] | Structural insights into the protein splicing mechanism of PI-SceI., Poland BW, Xu MQ, Quiocho FA, J Biol Chem. 2000 Jun 2;275(22):16408-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10828056 10828056] | ||
| - | [[Category: H(+)-transporting two-sector ATPase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Quiocho, F A.]] | [[Category: Quiocho, F A.]] | ||
[[Category: Xu, M Q.]] | [[Category: Xu, M Q.]] | ||
| - | [[Category: | + | [[Category: Endonuclease]] |
| - | [[Category: | + | [[Category: Mini-precursor]] |
| - | [[Category: | + | [[Category: Protein splicing]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:01:05 2008'' | |
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Revision as of 12:01, 2 May 2008
CRYSTAL STRUCTURE OF PI-SCEI MINIPRECURSOR
Overview
PI-SceI is a member of a class of proteins (inteins) that excise themselves from a precursor protein and in the process ligate the flanking protein sequences (exteins). We report here the 2.1-A resolution crystal structure of a PI-SceI miniprecursor (VMA29) containing 10 N-terminal extein residues and 4 C-terminal extein residues. Mutations at the N- and C-terminal splicing junctions, blocking in vivo protein splicing, allowed the miniprecursor to be purified and crystallized. The structure reveals both the N- and C-terminal scissile peptide bonds to be in distorted trans conformations (tau approximately 100 degrees ). Modeling of the wild-type PI-SceI based on the VMA29 structure indicates a large conformational change (movement of >9 A) must occur to allow transesterification to be completed. A zinc atom was discovered at the C-terminal splicing junction. Residues Cys(455), His(453), and Glu(80) along with a water molecule (Wat(53)) chelate the zinc atom. The crystal structure of VMA29 has captured the intein in its pre-spliced state.
About this Structure
1EF0 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural insights into the protein splicing mechanism of PI-SceI., Poland BW, Xu MQ, Quiocho FA, J Biol Chem. 2000 Jun 2;275(22):16408-13. PMID:10828056 Page seeded by OCA on Fri May 2 15:01:05 2008
