1efz

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[[Image:1efz.jpg|left|200px]]
[[Image:1efz.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1efz |SIZE=350|CAPTION= <scene name='initialview01'>1efz</scene>, resolution 2.00&Aring;
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The line below this paragraph, containing "STRUCTURE_1efz", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=PRF:7-DEAZA-7-AMINOMETHYL-GUANINE'>PRF</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Queuine_tRNA-ribosyltransferase Queuine tRNA-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.29 2.4.2.29] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1efz| PDB=1efz | SCENE= }}
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|RELATEDENTRY=[[1pud|1PUD]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1efz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1efz OCA], [http://www.ebi.ac.uk/pdbsum/1efz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1efz RCSB]</span>
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}}
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'''MUTAGENESIS AND CRYSTALLOGRAPHIC STUDIES OF ZYMOMONAS MOBILIS TRNA-GUANINE TRANSGLYCOSYLASE TO ELUCIDATE THE ROLE OF SERINE 103 FOR ENZYMATIC ACTIVITY'''
'''MUTAGENESIS AND CRYSTALLOGRAPHIC STUDIES OF ZYMOMONAS MOBILIS TRNA-GUANINE TRANSGLYCOSYLASE TO ELUCIDATE THE ROLE OF SERINE 103 FOR ENZYMATIC ACTIVITY'''
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[[Category: Reuter, K.]]
[[Category: Reuter, K.]]
[[Category: Stubbs, M T.]]
[[Category: Stubbs, M T.]]
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[[Category: glycosyltransferase]]
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[[Category: Glycosyltransferase]]
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[[Category: transferase]]
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[[Category: Transferase]]
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[[Category: trna-modifying enzyme]]
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[[Category: Trna-modifying enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:03:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:01:53 2008''
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Revision as of 12:03, 2 May 2008

Image:1efz.jpg

Template:STRUCTURE 1efz

MUTAGENESIS AND CRYSTALLOGRAPHIC STUDIES OF ZYMOMONAS MOBILIS TRNA-GUANINE TRANSGLYCOSYLASE TO ELUCIDATE THE ROLE OF SERINE 103 FOR ENZYMATIC ACTIVITY


Overview

The tRNA modifying enzyme tRNA-guanine transglycosylase (TGT) is involved in the exchange of guanine in the first position of the anticodon with preQ1 as part of the biosynthesis of the hypermodified base queuine (Q). Mutation of Ser90 to an alanine in Escherichia coli TGT leads to a dramatic reduction of enzymatic activity (Reuter, K. et al. (1994) Biochemistry 33, 7041-7046). To further clarify the role of this residue in the catalytic center, we have mutated the corresponding Ser103 of the crystallizable Zymomonas mobilis TGT into alanine. The crystal structure of a TGT(S103A)/preQ1 complex combined with biochemical data presented in this paper suggest that Ser103 is essential for substrate orientation in the TGT reaction.

About this Structure

1EFZ is a Single protein structure of sequence from Zymomonas mobilis. Full crystallographic information is available from OCA.

Reference

Mutagenesis and crystallographic studies of Zymomonas mobilis tRNA-guanine transglycosylase to elucidate the role of serine 103 for enzymatic activity., Gradler U, Ficner R, Garcia GA, Stubbs MT, Klebe G, Reuter K, FEBS Lett. 1999 Jul 2;454(1-2):142-6. PMID:10413112 Page seeded by OCA on Fri May 2 15:03:02 2008

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