7lva
From Proteopedia
(Difference between revisions)
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==Solution structure of the HIV-1 PBS-segment== | ==Solution structure of the HIV-1 PBS-segment== | ||
| - | <StructureSection load='7lva' size='340' side='right'caption='[[7lva]]' scene=''> | + | <StructureSection load='7lva' size='340' side='right'caption='[[7lva]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=7k1z 7k1z]. Full | + | <table><tr><td colspan='2'>[[7lva]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=7k1z 7k1z]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LVA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LVA FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lva OCA], [https://pdbe.org/7lva PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lva RCSB], [https://www.ebi.ac.uk/pdbsum/7lva PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lva ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lva OCA], [https://pdbe.org/7lva PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lva RCSB], [https://www.ebi.ac.uk/pdbsum/7lva PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lva ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | HIV-1 reverse transcription initiates at the primer binding site (PBS) in the viral genomic RNA (gRNA). Although the structure of the PBS-segment undergoes substantial rearrangement upon tRNALys3 annealing, the proper folding of the PBS-segment during gRNA packaging is important as it ensures loading of beneficial host factors. DHX9/RNA helicase A (RHA) is recruited to gRNA to enhance the processivity of reverse transcriptase. Because the molecular details of the interactions have yet to be defined, we solved the solution structure of the PBS-segment preferentially bound by RHA. Evidence is provided that PBS-segment adopts a previously undefined adenosine-rich three-way junction structure encompassing the primer activation stem (PAS), tRNA-like element (TLE) and tRNA annealing arm. Disruption of the PBS-segment three-way junction structure diminished reverse transcription products and led to reduced viral infectivity. Because of the existence of the tRNA annealing arm, the TLE and PAS form a bent helical structure that undergoes shape-dependent recognition by RHA double-stranded RNA binding domain 1 (dsRBD1). Mutagenesis and phylogenetic analyses provide evidence for conservation of the PBS-segment three-way junction structure that is preferentially bound by RHA in support of efficient reverse transcription, the hallmark step of HIV-1 replication. | ||
| + | |||
| + | The three-way junction structure of the HIV-1 PBS-segment binds host enzyme important for viral infectivity.,Song Z, Gremminger T, Singh G, Cheng Y, Li J, Qiu L, Ji J, Lange MJ, Zuo X, Chen SJ, Zou X, Boris-Lawrie K, Heng X Nucleic Acids Res. 2021 May 12. pii: 6274540. doi: 10.1093/nar/gkab342. PMID:33978756<ref>PMID:33978756</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7lva" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Heng X]] | + | [[Category: Heng, X]] |
| - | [[Category: Song Z]] | + | [[Category: Song, Z]] |
| + | [[Category: Primer binding site]] | ||
| + | [[Category: Reverse transcription]] | ||
| + | [[Category: Rna]] | ||
Revision as of 09:30, 26 May 2021
Solution structure of the HIV-1 PBS-segment
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