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| | <StructureSection load='1ais' size='340' side='right'caption='[[1ais]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='1ais' size='340' side='right'caption='[[1ais]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ais]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"pyrococcus_furiosus_subsp._woesei"_kanoksilapatham_et_al._2004 "pyrococcus furiosus subsp. woesei" kanoksilapatham et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AIS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AIS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ais]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"pyrococcus_furiosus_subsp._woesei"_kanoksilapatham_et_al._2004 "pyrococcus furiosus subsp. woesei" kanoksilapatham et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AIS FirstGlance]. <br> |
| | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5IU:5-IODO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>5IU</scene></td></tr> | | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5IU:5-IODO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>5IU</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ais FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ais OCA], [http://pdbe.org/1ais PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ais RCSB], [http://www.ebi.ac.uk/pdbsum/1ais PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ais ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ais FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ais OCA], [https://pdbe.org/1ais PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ais RCSB], [https://www.ebi.ac.uk/pdbsum/1ais PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ais ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TF2B_PYRWO TF2B_PYRWO]] Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB). [[http://www.uniprot.org/uniprot/TBP_PYRWO TBP_PYRWO]] General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation. | + | [[https://www.uniprot.org/uniprot/TF2B_PYRWO TF2B_PYRWO]] Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB). [[https://www.uniprot.org/uniprot/TBP_PYRWO TBP_PYRWO]] General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[TATA-Binding Protein|TATA-Binding Protein]] | + | *[[TATA-binding protein 3D structures|TATA-binding protein 3D structures]] |
| - | *[[Transcription initiation factor|Transcription initiation factor]] | + | *[[Transcription initiation factors 3D structures|Transcription initiation factors 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| Structural highlights
Function
[TF2B_PYRWO] Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB). [TBP_PYRWO] General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Archaea possess a basal transcriptional apparatus that resembles that of eukaryotes. Here we report the 2.1-A crystal structure of the archaeal transcription factor complex formed by the TATA-box-binding protein (TBP), the transcription factor IIB homolog, and a DNA target, all from the hyperthermophile Pyrococcus woesei. The overall fold of these two basal transcription factors is essentially the same as that of their eukaryotic counterparts. However, in comparison with the eukaryotic complexes, the archaeal TBP-DNA interface is more symmetrical, and in this structure the orientation of the preinitiation complex assembly on the promoter is inverted with respect to that seen in all crystal structures of comparable eukaryotic systems. This study of the structural details of an archaeal transcription factor complex presents the opportunity to examine the evolution of the basal eukaryotic transcriptional apparatus from a stereochemical viewpoint and to extend our understanding of the physical biochemistry of transcriptional initiation.
The 2.1-A crystal structure of an archaeal preinitiation complex: TATA-box-binding protein/transcription factor (II)B core/TATA-box.,Kosa PF, Ghosh G, DeDecker BS, Sigler PB Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6042-7. PMID:9177165[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kosa PF, Ghosh G, DeDecker BS, Sigler PB. The 2.1-A crystal structure of an archaeal preinitiation complex: TATA-box-binding protein/transcription factor (II)B core/TATA-box. Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6042-7. PMID:9177165
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