1ei4
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1ei4.gif|left|200px]] | [[Image:1ei4.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1ei4", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1ei4| PDB=1ei4 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''B-DNA DODECAMER CGCGAAT(TLC)CGCG WITH INCORPORATED [3.3.0]BICYCLO-ARABINO-THYMINE-5'-PHOSPHATE''' | '''B-DNA DODECAMER CGCGAAT(TLC)CGCG WITH INCORPORATED [3.3.0]BICYCLO-ARABINO-THYMINE-5'-PHOSPHATE''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EI4 OCA]. | |
==Reference== | ==Reference== | ||
Structural basis of cleavage by RNase H of hybrids of arabinonucleic acids and RNA., Minasov G, Teplova M, Nielsen P, Wengel J, Egli M, Biochemistry. 2000 Apr 4;39(13):3525-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10736151 10736151] | Structural basis of cleavage by RNase H of hybrids of arabinonucleic acids and RNA., Minasov G, Teplova M, Nielsen P, Wengel J, Egli M, Biochemistry. 2000 Apr 4;39(13):3525-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10736151 10736151] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Egli, M.]] | [[Category: Egli, M.]] | ||
[[Category: Minasov, G.]] | [[Category: Minasov, G.]] | ||
| Line 29: | Line 25: | ||
[[Category: Teplova, M.]] | [[Category: Teplova, M.]] | ||
[[Category: Wengel, J.]] | [[Category: Wengel, J.]] | ||
| - | [[Category: | + | [[Category: Modified b-dodecamer]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:07:41 2008'' | |
| - | + | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:07, 2 May 2008
B-DNA DODECAMER CGCGAAT(TLC)CGCG WITH INCORPORATED [3.3.0]BICYCLO-ARABINO-THYMINE-5'-PHOSPHATE
Overview
The origins of the substrate specificity of Escherichia coli RNase H1 (termed RNase H here), an enzyme that hydrolyzes the RNA strand of DNA-RNA hybrids, are not understood at present. Although the enzyme binds double-stranded RNA, no cleavage occurs with such duplexes [Lima, W. F., and Crooke, S. T. (1997) Biochemistry 36, 390]. Therefore, the hybrid substrates may not adopt a canonical A-form geometry. Furthermore, RNase H is exquisitely sensitive to chemical modification of the DNA strands in hybrid duplexes. This is particularly relevant to the RNase H-dependent pathway of antisense action. Thus, only very few of the modifications currently being evaluated as antisense therapeutics are tolerated by the enzyme, among them phosphorothioate DNA (PS-DNA). Recently, hybrids of RNA and arabinonucleic acid (ANA) as well as the 2'F-ANA analogue were shown to be substrates of RNase H [Damha, M. J., et al. (1998) J. Am. Chem. Soc. 120, 12976]. Using X-ray crystallography, we demonstrate here that ANA analogues, such as 2'F-ANA [Berger, I., et al. (1998) Nucleic Acids Res. 26, 2473] and [3.3.0]bicyclo-ANA (bc-ANA), may not be able to adopt sugar puckers that are compatible with pure A- or a B-form duplex geometries, but rather prefer the intermediate O4'-endo conformation. On the basis of the observed conformations of these ANA analogues in a DNA dodecamer duplex, we have modeled a duplex of an all-C3'-endo RNA strand and an all-O4'-endo 2'F-ANA strand. This duplex exhibits a minor groove width that is intermediate between that of A-form RNA and B-form DNA, a feature that may be exploited by the enzyme in differentiating between RNA duplexes and DNA-RNA hybrids. Therefore, the combination of the established structural and functional properties of ANA analogues helps settle existing controversies concerning the discrimination of substrates by RNase H. Knowlegde of the structure of an analogue that exhibits enhanced RNA affinity while not interfering with RNase H activity may prove helpful in the design of future antisense modifications.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structural basis of cleavage by RNase H of hybrids of arabinonucleic acids and RNA., Minasov G, Teplova M, Nielsen P, Wengel J, Egli M, Biochemistry. 2000 Apr 4;39(13):3525-32. PMID:10736151 Page seeded by OCA on Fri May 2 15:07:41 2008
