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7e4l
From Proteopedia
(Difference between revisions)
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==Conversion of pyrophosphate-dependent myo-inositol-1 kinase into myo-inositol-3 kinase by N78L/S89L mutation== | ==Conversion of pyrophosphate-dependent myo-inositol-1 kinase into myo-inositol-3 kinase by N78L/S89L mutation== | ||
| - | <StructureSection load='7e4l' size='340' side='right'caption='[[7e4l]]' scene=''> | + | <StructureSection load='7e4l' size='340' side='right'caption='[[7e4l]], [[Resolution|resolution]] 1.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E4L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E4L FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7e4l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thema Thema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E4L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E4L FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e4l OCA], [https://pdbe.org/7e4l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e4l RCSB], [https://www.ebi.ac.uk/pdbsum/7e4l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e4l ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MDN:METHYLENEDIPHOSPHONIC+ACID'>MDN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tmari_0412 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e4l OCA], [https://pdbe.org/7e4l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e4l RCSB], [https://www.ebi.ac.uk/pdbsum/7e4l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e4l ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Most kinases utilize ATP as a phosphate donor and phosphorylate a wide range of phosphate acceptors. An alternative phosphate donor is inorganic pyrophosphate (PPi), which costs only 1/1000 of ATP. To develop a method to engineer PPi-dependent kinases, we herein aimed to alter the product of PPi-dependent myo-inositol kinase from d-myo-inositol 1-phosphate to d-myo-inositol 3-phosphate. For this purpose, we introduced the myo-inositol recognition residues of the ATP-dependent myo-inositol-3-kinase into the PPi-dependent myo-inositol-1-kinase. This replacement was expected to change the 3D arrangements of myo-inositol in the active site and bring the hydroxyl group at the 3C position close to the catalytic residue. LC-MS and NMR analyses proved that the engineered enzyme successfully produced myo-inositol 3-phosphate from PPi and myo-inositol. | ||
| + | |||
| + | Altering the Phosphorylation Position of Pyrophosphate-Dependent myo-Inositol-1-Kinase Based on Its Crystal Structure.,Tashiro R, Sato T, Atomi H, Miki K, Fujihashi M ACS Chem Biol. 2021 Apr 20. doi: 10.1021/acschembio.0c00733. PMID:33877806<ref>PMID:33877806</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7e4l" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Fujihashi M]] | + | [[Category: Thema]] |
| - | [[Category: Miki K]] | + | [[Category: Fujihashi, M]] |
| - | [[Category: Tashiro R]] | + | [[Category: Miki, K]] |
| + | [[Category: Tashiro, R]] | ||
| + | [[Category: Pyrophosphate-dependent kinase]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 14:50, 2 June 2021
Conversion of pyrophosphate-dependent myo-inositol-1 kinase into myo-inositol-3 kinase by N78L/S89L mutation
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