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| | ==Solution structure of a bacterial immunoglobulin-like domain form a surface protein of Leptospira== | | ==Solution structure of a bacterial immunoglobulin-like domain form a surface protein of Leptospira== |
| - | <StructureSection load='2mqg' size='340' side='right' caption='[[2mqg]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2mqg' size='340' side='right'caption='[[2mqg]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mqg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leptospira_interrogans_pomona Leptospira interrogans pomona]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2mp7 2mp7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MQG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mqg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptospira_interrogans_pomona Leptospira interrogans pomona]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2mp7 2mp7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MQG FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ligB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44276 Leptospira interrogans pomona])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ligB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44276 Leptospira interrogans pomona])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqg OCA], [http://pdbe.org/2mqg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mqg RCSB], [http://www.ebi.ac.uk/pdbsum/2mqg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mqg ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqg OCA], [https://pdbe.org/2mqg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mqg RCSB], [https://www.ebi.ac.uk/pdbsum/2mqg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mqg ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Leptospira interrogans pomona]] | | [[Category: Leptospira interrogans pomona]] |
| | [[Category: Mei, S]] | | [[Category: Mei, S]] |
| | [[Category: Immune system]] | | [[Category: Immune system]] |
| Structural highlights
Publication Abstract from PubMed
Leptospiral immunoglobulin-like (Lig) proteins are surface proteins expressed in pathogenic strains of Leptospira. LigB, an outer membrane protein containing tandem repeats of bacterial Ig-like (Big) domains and a no-repeat tail, has been identified as a virulence factor involved in adhesion of pathogenic Leptospira interrogans to host cells. A Big domain of LigB, LigBCen2R, was reported previously to bind the GBD domain of fibronectin, suggesting its important role in leptospiral infections. In this study, we determined the solution structure of LigBCen2R by nuclear magnetic resonance (NMR) spectroscopy. LigBCen2R adopts a canonical immunoglobulin-like fold which is comprised of a beta-sandwich of ten strands in three sheets. We indicated that LigBCen2R is able to bind to Ca(2+) with a high affinity by isothermal titration calorimetry assay. NMR perturbation experiment identified a number of residues responsible for Ca(2+) binding. Structural comparison of it with other Big domains demonstrates that they share a similar fold pattern, but vary in some structural characters. Since Lig proteins play a vital role in the infection to host cells, our study will contribute a structural basis to understand the interactions between Leptospira and host cells. Proteins 2015; 83:195-200. (c) 2014 Wiley Periodicals, Inc.
Solution structure of a bacterial immunoglobulin-like domain of the outer membrane protein (LigB) from Leptospira.,Mei S, Zhang J, Zhang X, Tu X Proteins. 2015 Jan;83(1):195-200. doi: 10.1002/prot.24723. Epub 2014 Nov 21. PMID:25393078[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Mei S, Zhang J, Zhang X, Tu X. Solution structure of a bacterial immunoglobulin-like domain of the outer membrane protein (LigB) from Leptospira. Proteins. 2015 Jan;83(1):195-200. doi: 10.1002/prot.24723. Epub 2014 Nov 21. PMID:25393078 doi:http://dx.doi.org/10.1002/prot.24723
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