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| | ==NMR solution structure of the C-terminal domain of NisI, a lipoprotein from Lactococcus lactis which confers immunity against nisin== | | ==NMR solution structure of the C-terminal domain of NisI, a lipoprotein from Lactococcus lactis which confers immunity against nisin== |
| - | <StructureSection load='2n2e' size='340' side='right' caption='[[2n2e]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2n2e' size='340' side='right'caption='[[2n2e]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2n2e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"lactococcus_lactis_subsp._diacitilactis_(sic)"_(garvie_and_farrow_1982)_schleifer_et_al._1986 "lactococcus lactis subsp. diacitilactis (sic)" (garvie and farrow 1982) schleifer et al. 1986]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N2E FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2n2e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"lactococcus_lactis_subsp._diacitilactis_(sic)"_(garvie_and_farrow_1982)_schleifer_et_al._1986 "lactococcus lactis subsp. diacitilactis (sic)" (garvie and farrow 1982) schleifer et al. 1986]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N2E FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nisI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1360 "Lactococcus lactis subsp. diacitilactis (sic)" (Garvie and Farrow 1982) Schleifer et al. 1986])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nisI ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1360 "Lactococcus lactis subsp. diacitilactis (sic)" (Garvie and Farrow 1982) Schleifer et al. 1986])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n2e OCA], [http://pdbe.org/2n2e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n2e RCSB], [http://www.ebi.ac.uk/pdbsum/2n2e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n2e ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n2e OCA], [https://pdbe.org/2n2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n2e RCSB], [https://www.ebi.ac.uk/pdbsum/2n2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n2e ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NISI_LACLL NISI_LACLL]] Involved in immunity against exogenously supplied nisin. | + | [[https://www.uniprot.org/uniprot/NISI_LACLL NISI_LACLL]] Involved in immunity against exogenously supplied nisin. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Christ, N A]] | | [[Category: Christ, N A]] |
| | [[Category: Duchardt-Ferner, E]] | | [[Category: Duchardt-Ferner, E]] |
| Structural highlights
Function
[NISI_LACLL] Involved in immunity against exogenously supplied nisin.
Publication Abstract from PubMed
Many Gram-positive bacteria produce lantibiotics, genetically encoded and posttranslationally modified peptide antibiotics, which inhibit the growth of other Gram-positive bacteria. To protect themselves against their own lantibiotics these bacteria express a variety of immunity proteins including the LanI lipoproteins. The structural and mechanistic basis for LanI-mediated lantibiotic immunity is not yet understood. Lactococcus lactis produces the lantibiotic nisin, which is widely used as a food preservative. Its LanI protein NisI provides immunity against nisin but not against structurally very similar lantibiotics from other species such as subtilin from Bacillus subtilis. To understand the structural basis for LanI-mediated immunity and their specificity we investigated the structure of NisI. We found that NisI is a two-domain protein. Surprisingly, each of the two NisI domains has the same structure as the LanI protein from B. subtilis, SpaI, despite the lack of significant sequence homology. The two NisI domains and SpaI differ strongly in their surface properties and function. Additionally, SpaI-mediated lantibiotic immunity depends on the presence of a basic unstructured N-terminal region that tethers SpaI to the membrane. Such a region is absent from NisI. Instead, the N-terminal domain of NisI interacts with membranes but not with nisin. In contrast, the C-terminal domain specifically binds nisin and modulates the membrane affinity of the N-terminal domain. Thus, our results reveal an unexpected structural relationship between NisI and SpaI and shed light on the structural basis for LanI mediated lantibiotic immunity.
The Solution Structure of the Lantibiotic Immunity Protein NisI and Its Interactions with Nisin.,Hacker C, Christ NA, Duchardt-Ferner E, Korn S, Gobl C, Berninger L, Dusterhus S, Hellmich UA, Madl T, Kotter P, Entian KD, Wohnert J J Biol Chem. 2015 Nov 27;290(48):28869-86. doi: 10.1074/jbc.M115.679969. Epub, 2015 Oct 12. PMID:26459561[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hacker C, Christ NA, Duchardt-Ferner E, Korn S, Gobl C, Berninger L, Dusterhus S, Hellmich UA, Madl T, Kotter P, Entian KD, Wohnert J. The Solution Structure of the Lantibiotic Immunity Protein NisI and Its Interactions with Nisin. J Biol Chem. 2015 Nov 27;290(48):28869-86. doi: 10.1074/jbc.M115.679969. Epub, 2015 Oct 12. PMID:26459561 doi:http://dx.doi.org/10.1074/jbc.M115.679969
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