|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Solution structure of the meiosis-expressed gene 1 (Meig1)== | | ==Solution structure of the meiosis-expressed gene 1 (Meig1)== |
| - | <StructureSection load='2n2y' size='340' side='right' caption='[[2n2y]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2n2y' size='340' side='right'caption='[[2n2y]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2n2y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N2Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N2Y FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2n2y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N2Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N2Y FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Meig1, Meg1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Meig1, Meg1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n2y OCA], [http://pdbe.org/2n2y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n2y RCSB], [http://www.ebi.ac.uk/pdbsum/2n2y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n2y ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n2y OCA], [https://pdbe.org/2n2y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n2y RCSB], [https://www.ebi.ac.uk/pdbsum/2n2y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n2y ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MEIG1_MOUSE MEIG1_MOUSE]] Essential for spermiogenesis.<ref>PMID:1390336</ref> <ref>PMID:19805151</ref> | + | [[https://www.uniprot.org/uniprot/MEIG1_MOUSE MEIG1_MOUSE]] Essential for spermiogenesis.<ref>PMID:1390336</ref> <ref>PMID:19805151</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Lk3 transgenic mice]] | | [[Category: Lk3 transgenic mice]] |
| | [[Category: Buchwald, W A]] | | [[Category: Buchwald, W A]] |
| Structural highlights
Function
[MEIG1_MOUSE] Essential for spermiogenesis.[1] [2]
Publication Abstract from PubMed
The product of the meiosis-expressed gene 1 (MEIG1) is found in the cell bodies of spermatocytes and recruited to the manchette, a structure unique to elongating spermatids, by Parkin co-regulated gene (PACRG). This complex is essential for targeting cargo to the manchette during sperm flagellum assembly. Here we show that MEIG1 adopts a unique fold that provides a large surface for interacting with other proteins. We mutated 12 exposed and conserved amino acids and show that four of these mutations (W50A, K57E, F66A, Y68A) dramatically reduce binding to PACRG. These four amino acids form a contiguous hydrophobic patch on one end of the protein. Furthermore, each of these four mutations diminishes the ability of MEIG1 to stabilize PACRG when expressed in bacteria. Together these studies establish the unique structure and key interaction surface of MEIG1 and provide a framework to explore how MEIG1 recruits proteins to build the sperm tail.
Dissecting the structural basis of MEIG1 interaction with PACRG.,Li W, Walavalkar NM, Buchwald WA, Teves ME, Zhang L, Liu H, Bilinovich S, Peterson DL, Strauss Iii JF, Williams DC Jr, Zhang Z Sci Rep. 2016 Jan 4;6:18278. doi: 10.1038/srep18278. PMID:26726850[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Don J, Wolgemuth DJ. Identification and characterization of the regulated pattern of expression of a novel mouse gene, meg1, during the meiotic cell cycle. Cell Growth Differ. 1992 Aug;3(8):495-505. PMID:1390336
- ↑ Zhang Z, Shen X, Gude DR, Wilkinson BM, Justice MJ, Flickinger CJ, Herr JC, Eddy EM, Strauss JF 3rd. MEIG1 is essential for spermiogenesis in mice. Proc Natl Acad Sci U S A. 2009 Oct 6;106(40):17055-60. doi:, 10.1073/pnas.0906414106. Epub 2009 Sep 17. PMID:19805151 doi:http://dx.doi.org/10.1073/pnas.0906414106
- ↑ Li W, Walavalkar NM, Buchwald WA, Teves ME, Zhang L, Liu H, Bilinovich S, Peterson DL, Strauss Iii JF, Williams DC Jr, Zhang Z. Dissecting the structural basis of MEIG1 interaction with PACRG. Sci Rep. 2016 Jan 4;6:18278. doi: 10.1038/srep18278. PMID:26726850 doi:http://dx.doi.org/10.1038/srep18278
|
