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| | ==Solution Structure of Lacticin Q== | | ==Solution Structure of Lacticin Q== |
| - | <StructureSection load='2n8p' size='340' side='right' caption='[[2n8p]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2n8p' size='340' side='right'caption='[[2n8p]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2n8p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_lactis"_lister_1873 "bacterium lactis" lister 1873]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N8P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2n8p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_lactis"_lister_1873 "bacterium lactis" lister 1873]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N8P FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2n8o|2n8o]]</td></tr> | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2n8o|2n8o]]</div></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lnqQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 "Bacterium lactis" Lister 1873])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lnqQ ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 "Bacterium lactis" Lister 1873])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8p OCA], [http://pdbe.org/2n8p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n8p RCSB], [http://www.ebi.ac.uk/pdbsum/2n8p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8p ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8p OCA], [https://pdbe.org/2n8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n8p RCSB], [https://www.ebi.ac.uk/pdbsum/2n8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8p ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Bacterium lactis lister 1873]] | | [[Category: Bacterium lactis lister 1873]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Acedo, J Z]] | | [[Category: Acedo, J Z]] |
| | [[Category: Belkum, M J.van]] | | [[Category: Belkum, M J.van]] |
| Structural highlights
Publication Abstract from PubMed
Lacticin Q (LnqQ) and aureocin A53 (AucA) are leaderless bacteriocins from Lactococcus lactis QU5 and Staphylococcus aureus A53, respectively. These bacteriocins are characterized by the absence of an N-terminal leader sequence and are active against a broad range of Gram-positive bacteria. LnqQ and AucA consist of 53 and 51 amino acids, respectively, and have 47% identical sequences. In this study, their three-dimensional structures were elucidated using solution nuclear magnetic resonance and were shown to consist of four alpha-helices that assume a very similar compact, globular overall fold (root-mean-square deviation of 1.7 A) with a highly cationic surface and a hydrophobic core. The structures of LnqQ and AucA resemble the shorter two-component leaderless bacteriocins, enterocins 7A and 7B, despite having low levels of sequence identity. Homology modeling revealed that the observed structural motif may be shared among leaderless bacteriocins with broad-spectrum activity against Gram-positive organisms. The elucidated structures of LnqQ and AucA also exhibit some resemblance to circular bacteriocins. Despite their similar overall fold, inhibition studies showed that LnqQ and AucA have different antimicrobial potency against the Gram-positive strains tested, suggesting that sequence disparities play a crucial role in their mechanisms of action.
Nuclear Magnetic Resonance Solution Structures of Lacticin Q and Aureocin A53 Reveal a Structural Motif Conserved among Leaderless Bacteriocins with Broad-Spectrum Activity.,Acedo JZ, van Belkum MJ, Lohans CT, Towle KM, Miskolzie M, Vederas JC Biochemistry. 2016 Feb 2;55(4):733-42. doi: 10.1021/acs.biochem.5b01306. Epub, 2016 Jan 15. PMID:26771761[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Acedo JZ, van Belkum MJ, Lohans CT, Towle KM, Miskolzie M, Vederas JC. Nuclear Magnetic Resonance Solution Structures of Lacticin Q and Aureocin A53 Reveal a Structural Motif Conserved among Leaderless Bacteriocins with Broad-Spectrum Activity. Biochemistry. 2016 Feb 2;55(4):733-42. doi: 10.1021/acs.biochem.5b01306. Epub, 2016 Jan 15. PMID:26771761 doi:http://dx.doi.org/10.1021/acs.biochem.5b01306
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