1el2

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[[Image:1el2.gif|left|200px]]
[[Image:1el2.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1el2 |SIZE=350|CAPTION= <scene name='initialview01'>1el2</scene>
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The line below this paragraph, containing "STRUCTURE_1el2", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=5CM:5-METHYL-2&#39;-DEOXY-CYTIDINE-5&#39;-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=DU:2&#39;-DEOXYURIDINE-5&#39;-MONOPHOSPHATE'>DU</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1el2| PDB=1el2 | SCENE= }}
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|RELATEDENTRY=[[1eln|1ELN]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1el2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1el2 OCA], [http://www.ebi.ac.uk/pdbsum/1el2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1el2 RCSB]</span>
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}}
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'''SOLUTION STRUCTURE OF A MODIFIED HUMAN TELOMERE FRAGMENT (STRUCTURE "A")'''
'''SOLUTION STRUCTURE OF A MODIFIED HUMAN TELOMERE FRAGMENT (STRUCTURE "A")'''
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==About this Structure==
==About this Structure==
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1EL2 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EL2 OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EL2 OCA].
==Reference==
==Reference==
The solution structure and internal motions of a fragment of the cytidine-rich strand of the human telomere., Phan AT, Gueron M, Leroy JL, J Mol Biol. 2000 May 26;299(1):123-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10860727 10860727]
The solution structure and internal motions of a fragment of the cytidine-rich strand of the human telomere., Phan AT, Gueron M, Leroy JL, J Mol Biol. 2000 May 26;299(1):123-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10860727 10860727]
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[[Category: Protein complex]]
 
[[Category: Gueron, M.]]
[[Category: Gueron, M.]]
[[Category: Leroy, J L.]]
[[Category: Leroy, J L.]]
[[Category: Phan, A T.]]
[[Category: Phan, A T.]]
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[[Category: dna solution structure]]
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[[Category: Dna solution structure]]
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[[Category: i-motif]]
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[[Category: I-motif]]
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[[Category: interacting loop]]
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[[Category: Interacting loop]]
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[[Category: loop motion]]
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[[Category: Loop motion]]
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[[Category: nmr]]
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[[Category: Nmr]]
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[[Category: telomere]]
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[[Category: Telomere]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:13:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:04:46 2008''
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Revision as of 12:13, 2 May 2008

Image:1el2.gif

Template:STRUCTURE 1el2

SOLUTION STRUCTURE OF A MODIFIED HUMAN TELOMERE FRAGMENT (STRUCTURE "A")


Overview

We present the solution structure of d(CCCTA2CCCTA2CCCTA2CCCT), a fragment of the vertebrate telomere which folds intramolecularly. The four cytidine stretches form an i-motif which includes six intercalated C.C+ pairs and terminates with the cytidines at the 5' extremity of each stretch. Above, the second TA2 linker loops across one of the narrow grooves, while at the bottom, the first and third linkers loop across the wide grooves. At 30 degrees C, the spectra of the first and third linkers are quasi-degenerate. Severe broadening at lower temperature indicates that this results from motional averaging between at least two structures of each bottom loop, and makes it impossible to solve the configuration of the bottom loops directly, in contrast to the rest of the structure. We therefore turned to the modified sequence d(CCCTA(2)5MCCCTA2CCCUA2CCCT) in which the two base substitutions (underlined) break the quasi-symmetry between linkers 1 and 3. The three loops follow approximately the hairpin "second pattern" of Hilbers. In the first loop, T4 is in the syn orientation, whereas its analog in the third loop, U16, oriented anti, is in a central location, where it interacts with bases of both loops, thus contributing to their tight association. The only motion is a syn/anti flip of A18 in the third loop. Returning to the telomere fragment, we show that each of the bottom loops switches between the structures identified in the first and third loops of the modified structure. The motions are concerted, and the resulting configurations of the bottom loop cluster present a bulge to either right (T4 syn) or left (T16 syn).

About this Structure

Full crystallographic information is available from OCA.

Reference

The solution structure and internal motions of a fragment of the cytidine-rich strand of the human telomere., Phan AT, Gueron M, Leroy JL, J Mol Biol. 2000 May 26;299(1):123-44. PMID:10860727 Page seeded by OCA on Fri May 2 15:13:51 2008

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