1emt
From Proteopedia
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[[Image:1emt.gif|left|200px]] | [[Image:1emt.gif|left|200px]] | ||
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'''FAB ANTIBODY FRAGMENT OF AN C60 ANTIFULLERENE ANTIBODY''' | '''FAB ANTIBODY FRAGMENT OF AN C60 ANTIFULLERENE ANTIBODY''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMT OCA]. | |
==Reference== | ==Reference== | ||
X-ray crystal structure of an anti-Buckminsterfullerene antibody fab fragment: biomolecular recognition of C(60)., Braden BC, Goldbaum FA, Chen BX, Kirschner AN, Wilson SR, Erlanger BF, Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12193-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11035793 11035793] | X-ray crystal structure of an anti-Buckminsterfullerene antibody fab fragment: biomolecular recognition of C(60)., Braden BC, Goldbaum FA, Chen BX, Kirschner AN, Wilson SR, Erlanger BF, Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12193-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11035793 11035793] | ||
| - | [[Category: Mus musculus]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Braden, B C.]] | [[Category: Braden, B C.]] | ||
[[Category: Chen, B X.]] | [[Category: Chen, B X.]] | ||
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[[Category: Kirschner, A N.]] | [[Category: Kirschner, A N.]] | ||
[[Category: Wilson, S R.]] | [[Category: Wilson, S R.]] | ||
| - | [[Category: | + | [[Category: Anti-fullerene antibody]] |
| - | [[Category: | + | [[Category: Nanotube]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:17:32 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:17, 2 May 2008
FAB ANTIBODY FRAGMENT OF AN C60 ANTIFULLERENE ANTIBODY
Overview
We have prepared a monoclonal Buckminsterfullerene specific antibody and report the sequences of its light and heavy chains. We also show, by x-ray crystallographic analysis of the Fab fragment and by model building, that the fullerene binding site is formed by the interface of the antibody light and heavy chains. Shape-complementary clustering of hydrophobic amino acids, several of which participate in putative stacking interactions with fullerene, form the binding site. Moreover, an induced fit mechanism appears to participate in the fullerene binding process. Affinity of the antibody-fullerene complex is 22 nM as measured by competitive binding. These findings should be applicable not only to the use of antibodies to assay and direct potential fullerene-based drug design but could also lead to new methodologies for the production of fullerene derivatives and nanotubes as well.
About this Structure
Full crystallographic information is available from OCA.
Reference
X-ray crystal structure of an anti-Buckminsterfullerene antibody fab fragment: biomolecular recognition of C(60)., Braden BC, Goldbaum FA, Chen BX, Kirschner AN, Wilson SR, Erlanger BF, Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12193-7. PMID:11035793 Page seeded by OCA on Fri May 2 15:17:32 2008
