7dog

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==Crystal structure of a nuclease and capping domain of SbcD from Staphylococcus aureus==
==Crystal structure of a nuclease and capping domain of SbcD from Staphylococcus aureus==
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<StructureSection load='7dog' size='340' side='right'caption='[[7dog]]' scene=''>
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<StructureSection load='7dog' size='340' side='right'caption='[[7dog]], [[Resolution|resolution]] 2.91&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DOG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DOG FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7dog]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DOG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DOG FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dog OCA], [https://pdbe.org/7dog PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dog RCSB], [https://www.ebi.ac.uk/pdbsum/7dog PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dog ProSAT]</span></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sbcD, SAV1345 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dog OCA], [https://pdbe.org/7dog PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dog RCSB], [https://www.ebi.ac.uk/pdbsum/7dog PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dog ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/SBCD_STAAM SBCD_STAAM]] SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The SbcCD complex is an essential component of the DNA double-strand break (DSB) repair system in bacteria. The bacterial SbcCD complex recognizes and cleaves the DNA ends in DSBs by ATP-dependent endo- and exonuclease activities as an early step of the DNA repair process. SbcD consists of nuclease, capping, and helix-loop-helix domains. Here, we present the crystal structure of a SbcD fragment from Staphylococcus aureus, which contained nuclease and capping domains, at a resolution of 2.9 A. This structure shows a dimeric assembly similar to that of the corresponding domains of SbcD from Escherichia coli. The S. aureus SbcD fragment exhibited endonuclease activities on supercoiled DNA and exonuclease activity on linear and nicked DNA. This study contributes to the understanding of the molecular basis for how bacteria can resist sterilizing treatment, causing DNA damage.
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Crystal structure of the nuclease and capping domain of SbcD from Staphylococcus aureus.,Lee J, Jo I, Ahn J, Hong S, Jeong S, Kwon A, Ha NC J Microbiol. 2021 Apr 20. pii: 10.1007/s12275-021-1012-0. doi:, 10.1007/s12275-021-1012-0. PMID:33877576<ref>PMID:33877576</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7dog" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Ha N-C]]
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[[Category: Staam]]
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[[Category: Lee J]]
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[[Category: Ha, N C]]
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[[Category: Lee, J]]
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[[Category: Dna nuclease]]
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[[Category: Dna repair]]
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[[Category: Endonuclease]]
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[[Category: Exonuclease]]
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[[Category: Hydrolase]]

Revision as of 14:58, 17 June 2021

Crystal structure of a nuclease and capping domain of SbcD from Staphylococcus aureus

PDB ID 7dog

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