7kbo
From Proteopedia
(Difference between revisions)
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==Reverse Transcriptase Diabody with S82bC, R83T Mutations Crystallized in C2== | ==Reverse Transcriptase Diabody with S82bC, R83T Mutations Crystallized in C2== | ||
| - | <StructureSection load='7kbo' size='340' side='right'caption='[[7kbo]]' scene=''> | + | <StructureSection load='7kbo' size='340' side='right'caption='[[7kbo]], [[Resolution|resolution]] 2.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KBO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7kbo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KBO FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kbo OCA], [https://pdbe.org/7kbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kbo RCSB], [https://www.ebi.ac.uk/pdbsum/7kbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kbo ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">scFv ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kbo OCA], [https://pdbe.org/7kbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kbo RCSB], [https://www.ebi.ac.uk/pdbsum/7kbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kbo ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | This work presents a method for introducing synthetic symmetry into protein crystallization samples using an antibody fragment termed a diabody (Dab). These Dabs contain two target binding sites, and engineered disulfide bonds have been included to modulate Dab flexibility. The impacts of Dab engineering have been observed through assessment of thermal stability, small-angle X-ray scattering, and high-resolution crystal structures. Complexes between the engineered Dabs and HIV-1 reverse transcriptase (RT) bound to a high-affinity DNA aptamer were also generated to explore the capacity of engineered Dabs to enable the crystallization of bound target proteins. This strategy increased the crystallization hit frequency obtained for RT-aptamer, and the structure of a Dab-RT-aptamer complex was determined to 3.0-A resolution. Introduction of synthetic symmetry using a Dab could be a broadly applicable strategy, especially when monoclonal antibodies for a target have previously been identified. | ||
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| + | Co-crystallization with diabodies: a case study for the introduction of synthetic symmetry.,Chesterman C, Arnold E Structure. 2021 Feb 23. pii: S0969-2126(21)00045-9. doi:, 10.1016/j.str.2021.02.001. PMID:33636101<ref>PMID:33636101</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7kbo" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Antibody 3D structures|Antibody 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Human]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Arnold E]] | + | [[Category: Arnold, E]] |
| - | [[Category: Chesterman C]] | + | [[Category: Chesterman, C]] |
| + | [[Category: Diabody]] | ||
| + | [[Category: Immunoglobin]] | ||
| + | [[Category: Protein binding]] | ||
| + | [[Category: Reverse transcriptase]] | ||
Revision as of 14:59, 17 June 2021
Reverse Transcriptase Diabody with S82bC, R83T Mutations Crystallized in C2
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