7bnw
From Proteopedia
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==Llama nanobody nb18 raised against GldL from Flavobacterium jonhsoniae== | ==Llama nanobody nb18 raised against GldL from Flavobacterium jonhsoniae== | ||
| - | <StructureSection load='7bnw' size='340' side='right'caption='[[7bnw]]' scene=''> | + | <StructureSection load='7bnw' size='340' side='right'caption='[[7bnw]], [[Resolution|resolution]] 2.59Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BNW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BNW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7bnw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BNW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BNW FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bnw OCA], [https://pdbe.org/7bnw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bnw RCSB], [https://www.ebi.ac.uk/pdbsum/7bnw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bnw ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bnw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bnw OCA], [https://pdbe.org/7bnw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bnw RCSB], [https://www.ebi.ac.uk/pdbsum/7bnw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bnw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | GldL is an inner-membrane protein that is essential for the function of the type IX secretion system (T9SS) in Flavobacterium johnsoniae. The complex that it forms with GldM is supposed to act as a new rotary motor involved in the gliding motility of the bacterium. In the context of structural studies of GldL to gain information on the assembly and function of the T9SS, two camelid nanobodies were selected, produced and purified. Their interaction with the cytoplasmic domain of GldL was characterized and their crystal structures were solved. These nanobodies will be used as crystallization chaperones to help in the crystallization of the cytoplasmic domain of GldL and could also help to solve the structure of the complex using molecular replacement. | ||
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| + | Crystal structures of two camelid nanobodies raised against GldL, a component of the type IX secretion system from Flavobacterium johnsoniae.,Trinh TTN, Gaubert A, Melani P, Cambillau C, Roussel A, Leone P Acta Crystallogr F Struct Biol Commun. 2021 Jun 1;77(Pt 6):171-176. doi:, 10.1107/S2053230X21005185. Epub 2021 May 24. PMID:34100775<ref>PMID:34100775</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7bnw" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Camelus glama]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Cambillau C]] | + | [[Category: Cambillau, C]] |
| - | [[Category: Leone P]] | + | [[Category: Leone, P]] |
| - | [[Category: Trinh | + | [[Category: Trinh, N T]] |
| + | [[Category: Immune system]] | ||
| + | [[Category: Nanobody]] | ||
| + | [[Category: Type ix secretion system]] | ||
Revision as of 07:47, 25 June 2021
Llama nanobody nb18 raised against GldL from Flavobacterium jonhsoniae
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