Journal:Acta Cryst D:S2059798319009008
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<b>Molecular Tour</b><br> | <b>Molecular Tour</b><br> | ||
| - | Solving a protein crystal structure without a homologous template involves experimental phasing. Ultimately, this requires the incorporation of heavy atoms into the lattice of the protein crystal. However, this process can be very laborious as suitable heavy atom compounds and conditions are found empirically through screening. A method to efficiently derivatize crystals was developed, by performing a Random Microseed Matrix screen (rMMS) in the presence of I3C. | + | Solving a protein crystal structure without a homologous template involves experimental phasing. Ultimately, this requires the incorporation of heavy atoms into the lattice of the protein crystal. However, this process can be very laborious as suitable heavy atom compounds and conditions are found empirically through screening. A method to efficiently derivatize crystals was developed, by performing a Random Microseed Matrix screen (rMMS) in the presence of 5-amino-2,4,6-triiodoisophthalic acid (I3C). |
| - | In a random microseed matrix, a crystal or crystalline precipitate is crushed up, diluted and added to new crystallization conditions from a sparse matrix screen. This often generates new crystallization where crystals can grow. I3C | + | In a random microseed matrix, a crystal or crystalline precipitate is crushed up, diluted and added to new crystallization conditions from a sparse matrix screen. This often generates new crystallization where crystals can grow. I3C is compatible with many crystallization reagents and provides a large phasing power. |
In the two test proteins, the <scene name='81/819238/Cv/2'>Orf11 NTD</scene> from ''Staphylococcus'' phage P68 and <scene name='81/819238/Cv/3'>Hen Egg White Lysozyme</scene>, the rMMS technique in the presence of I3C generated numerous new conditions where derivatized crystals could grow. The I3C molecules in the lattice were sufficient to completely solve the structure using anomalous phasing methods. | In the two test proteins, the <scene name='81/819238/Cv/2'>Orf11 NTD</scene> from ''Staphylococcus'' phage P68 and <scene name='81/819238/Cv/3'>Hen Egg White Lysozyme</scene>, the rMMS technique in the presence of I3C generated numerous new conditions where derivatized crystals could grow. The I3C molecules in the lattice were sufficient to completely solve the structure using anomalous phasing methods. | ||
Revision as of 19:16, 27 June 2021
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