6rj9

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<SX load='6rj9' size='340' side='right' viewer='molstar' caption='[[6rj9]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
<SX load='6rj9' size='340' side='right' viewer='molstar' caption='[[6rj9]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6rj9]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/ ], [http://en.wikipedia.org/wiki/Atcc_19258 Atcc 19258] and [http://en.wikipedia.org/wiki/Streptococcus_phage_d1811 Streptococcus phage d1811]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6RJ9 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6rj9]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/ ], [https://en.wikipedia.org/wiki/Atcc_19258 Atcc 19258] and [https://en.wikipedia.org/wiki/Streptococcus_phage_d1811 Streptococcus phage d1811]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RJ9 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6rja|6rja]], [[6rjd|6rjd]], [[6rjg|6rjg]]</td></tr>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6rja|6rja]], [[6rjd|6rjd]], [[6rjg|6rjg]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">D1811_026 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2108111 Streptococcus phage D1811]), cas9, CDA68_00396 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1308 ATCC 19258])</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">D1811_026 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2108111 Streptococcus phage D1811]), cas9-1, csn1, STER_0709 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1308 ATCC 19258])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6rj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rj9 OCA], [http://pdbe.org/6rj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rj9 RCSB], [http://www.ebi.ac.uk/pdbsum/6rj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rj9 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rj9 OCA], [https://pdbe.org/6rj9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rj9 RCSB], [https://www.ebi.ac.uk/pdbsum/6rj9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rj9 ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/CAS9A_STRTD CAS9A_STRTD]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity (By similarity). Cuts target DNA when Cas9 and gRNAs are mixed.[HAMAP-Rule:MF_01480]<ref>PMID:24270795</ref>
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<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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</div>
<div class="pdbe-citations 6rj9" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 6rj9" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>

Revision as of 03:42, 2 July 2021

Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 monomeric assembly.

6rj9, resolution 3.20Å

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