1eqn
From Proteopedia
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'''E.COLI PRIMASE CATALYTIC CORE''' | '''E.COLI PRIMASE CATALYTIC CORE''' | ||
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[[Category: McInerney, P.]] | [[Category: McInerney, P.]] | ||
[[Category: Podobnik, M.]] | [[Category: Podobnik, M.]] | ||
| - | [[Category: | + | [[Category: Rossmann fold]] |
| - | [[Category: | + | [[Category: Toprim domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:24:59 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:25, 2 May 2008
E.COLI PRIMASE CATALYTIC CORE
Overview
Primases synthesize short RNA strands on single-stranded DNA templates, thereby generating the hybrid duplexes required for the initiation of synthesis by DNA polymerases. We present the crystal structure of the catalytic unit of a primase enzyme, that of a approximately 320 residue fragment of Escherichia coli primase, determined at 2.9 A resolution. Central to the catalytic unit is a TOPRIM domain that is strikingly similar in its structure to that of corresponding domains in DNA topoisomerases, but is unrelated to the catalytic centers of other DNA or RNA polymerases. The catalytic domain of primase is crescent-shaped, and the concave face of the crescent is predicted to accommodate about 10 base-pairs of RNA-DNA duplex in a loose interaction, thereby limiting processivity.
About this Structure
1EQN is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
A TOPRIM domain in the crystal structure of the catalytic core of Escherichia coli primase confirms a structural link to DNA topoisomerases., Podobnik M, McInerney P, O'Donnell M, Kuriyan J, J Mol Biol. 2000 Jul 7;300(2):353-62. PMID:10873470 Page seeded by OCA on Fri May 2 15:24:59 2008
