7nn6
From Proteopedia
(Difference between revisions)
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==periplasmic domain of Vibrio cholerae ToxR== | ==periplasmic domain of Vibrio cholerae ToxR== | ||
| - | <StructureSection load='7nn6' size='340' side='right'caption='[[7nn6]]' scene=''> | + | <StructureSection load='7nn6' size='340' side='right'caption='[[7nn6]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full | + | <table><tr><td colspan='2'>[[7nn6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NN6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NN6 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nn6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nn6 OCA], [https://pdbe.org/7nn6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nn6 RCSB], [https://www.ebi.ac.uk/pdbsum/7nn6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nn6 ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">toxR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nn6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nn6 OCA], [https://pdbe.org/7nn6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nn6 RCSB], [https://www.ebi.ac.uk/pdbsum/7nn6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nn6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The transmembrane protein ToxR plays a key role in the virulence expression system of Vibrio cholerae. The activity of ToxR is dependent on its periplasmic sensor domain (ToxRp) and on the inner membrane protein ToxS. Herein, we present the Nuclear Magnetic Resonance NMR solution structure of the sensory ToxRp containing an intramolecular disulfide bond. The presented structural and dynamic experiments with reduced and oxidized ToxRp propose an explanation for the increased proteolytic sensitivity of reduced ToxR. Additionally, for the first time, we could identify the formation of a strong heterodimer complex between the periplasmic domains of ToxR and ToxS in solution. NMR interaction studies reveal that binding of ToxS is not dependent on the redox state of ToxR cysteines, and formed complexes are structurally similar. By monitoring the proteolytic cleavage of ToxRp with NMR, we additionally provide a direct evidence of ToxS protective function. Taken together our results suggest that ToxR activity is regulated by its stability which is, on the one hand, dependent on the redox states of its cysteines, influencing the stability of its fold, and on the other hand, on its interaction with ToxS, which binds independent on the cysteines and acts as a protection against proteases. | ||
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| + | The periplasmic domains of Vibriocholerae ToxR and ToxS are forming a strong heterodimeric complex independent on the redox state of ToxR cysteines.,Gubensak N, Wagner GE, Schrank E, Falsone FS, Berger TMI, Pavkov-Keller T, Reidl J, Zangger K Mol Microbiol. 2020 Dec 23. doi: 10.1111/mmi.14673. PMID:33368680<ref>PMID:33368680</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7nn6" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bacillo virgola del koch trevisan 1884]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Gubensaek N]] | + | [[Category: Gubensaek, N]] |
| - | [[Category: Wagner | + | [[Category: Wagner, G E]] |
| - | [[Category: Zangger K]] | + | [[Category: Zangger, K]] |
| + | [[Category: Transcription]] | ||
| + | [[Category: Vibrio cholerae trancription factor sensory domain]] | ||
Revision as of 03:51, 2 July 2021
periplasmic domain of Vibrio cholerae ToxR
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