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| | ==Crystal structure of human epsinR ENTH domain== | | ==Crystal structure of human epsinR ENTH domain== |
| - | <StructureSection load='2qy7' size='340' side='right' caption='[[2qy7]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='2qy7' size='340' side='right'caption='[[2qy7]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2qy7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QY7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QY7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2qy7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QY7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QY7 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CLINT1, ENTH, EPN4, EPNR, KIAA0171 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CLINT1, ENTH, EPN4, EPNR, KIAA0171 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qy7 OCA], [http://pdbe.org/2qy7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qy7 RCSB], [http://www.ebi.ac.uk/pdbsum/2qy7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2qy7 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qy7 OCA], [https://pdbe.org/2qy7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qy7 RCSB], [https://www.ebi.ac.uk/pdbsum/2qy7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qy7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/EPN4_HUMAN EPN4_HUMAN]] Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly.<ref>PMID:12429846</ref> <ref>PMID:12538641</ref> | + | [[https://www.uniprot.org/uniprot/EPN4_HUMAN EPN4_HUMAN]] Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly.<ref>PMID:12429846</ref> <ref>PMID:12538641</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </div> | | </div> |
| | <div class="pdbe-citations 2qy7" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 2qy7" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Epsin|Epsin]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Human]] | | [[Category: Human]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Collins, B M]] | | [[Category: Collins, B M]] |
| | [[Category: McCoy, A J]] | | [[Category: McCoy, A J]] |
| Structural highlights
Function
[EPN4_HUMAN] Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Soluble NSF attachment protein receptors (SNAREs) are type II transmembrane proteins that have critical roles in providing the specificity and energy for transport-vesicle fusion and must therefore be correctly partitioned between vesicle and organelle membranes. Like all other cargo, SNAREs need to be sorted into the forming vesicles by direct interaction with components of the vesicles' coats. Here we characterize the molecular details governing the sorting of a SNARE into clathrin-coated vesicles, namely the direct recognition of the three-helical bundle H(abc) domain of the mouse SNARE Vti1b by the human clathrin adaptor epsinR (EPNR, also known as CLINT1). Structures of each domain and of their complex show that this interaction (dissociation constant 22 muM) is mediated by surface patches composed of approximately 15 residues each, the topographies of which are dependent on each domain's overall fold. Disruption of the interface with point mutations abolishes the interaction in vitro and causes Vti1b to become relocalized to late endosomes and lysosomes. This new class of highly specific, surface-surface interaction between the clathrin coat component and the cargo is distinct from the widely observed binding of short, linear cargo motifs by the assembly polypeptide (AP) complex and GGA adaptors and is therefore not vulnerable to competition from standard motif-containing cargoes for incorporation into clathrin-coated vesicles. We propose that conceptually similar but mechanistically different interactions will direct the post-Golgi trafficking of many SNAREs.
A SNARE-adaptor interaction is a new mode of cargo recognition in clathrin-coated vesicles.,Miller SE, Collins BM, McCoy AJ, Robinson MS, Owen DJ Nature. 2007 Nov 22;450(7169):570-4. PMID:18033301[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kalthoff C, Groos S, Kohl R, Mahrhold S, Ungewickell EJ. Clint: a novel clathrin-binding ENTH-domain protein at the Golgi. Mol Biol Cell. 2002 Nov;13(11):4060-73. PMID:12429846 doi:http://dx.doi.org/10.1091/mbc.E02-03-0171
- ↑ Mills IG, Praefcke GJ, Vallis Y, Peter BJ, Olesen LE, Gallop JL, Butler PJ, Evans PR, McMahon HT. EpsinR: an AP1/clathrin interacting protein involved in vesicle trafficking. J Cell Biol. 2003 Jan 20;160(2):213-22. Epub 2003 Jan 21. PMID:12538641 doi:http://dx.doi.org/10.1083/jcb.200208023
- ↑ Miller SE, Collins BM, McCoy AJ, Robinson MS, Owen DJ. A SNARE-adaptor interaction is a new mode of cargo recognition in clathrin-coated vesicles. Nature. 2007 Nov 22;450(7169):570-4. PMID:18033301 doi:http://dx.doi.org/nature06353
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