1erd
From Proteopedia
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'''THE NMR SOLUTION STRUCTURE OF THE PHEROMONE ER-2 FROM THE CILIATED PROTOZOAN EUPLOTES RAIKOVI''' | '''THE NMR SOLUTION STRUCTURE OF THE PHEROMONE ER-2 FROM THE CILIATED PROTOZOAN EUPLOTES RAIKOVI''' | ||
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[[Category: Szyperski, T.]] | [[Category: Szyperski, T.]] | ||
[[Category: Wuthrich, K.]] | [[Category: Wuthrich, K.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:25:58 2008'' | |
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Revision as of 12:25, 2 May 2008
THE NMR SOLUTION STRUCTURE OF THE PHEROMONE ER-2 FROM THE CILIATED PROTOZOAN EUPLOTES RAIKOVI
Overview
The NMR structure of the pheromone Er-2 from the ciliated protozoan Euplotes raikovi has been determined in aqueous solution. The structure of this 40-residue protein was calculated with the distance geometry program DIANA from 621 distance constraints and 89 dihedral angle constraints; the program OPAL was employed for the energy minimization. For a group of 20 conformers used to characterize the solution structure, the average pairwise RMS deviation from the mean structure calculated for the backbone heavy atoms N, C alpha, and C' of residues 3-37 was 0.31 A. The molecular architecture is dominated by an up-down-up bundle of 3 short helices of residues 5-11, 14-20, and 23-33, which is similar to the structures of the homologous pheromones Er-1 and Er-10. Novel structural features include a well-defined N-cap on the first helix, a 1-residue deletion in the second helix resulting in the formation of a 3(10)-helix rather than an alpha-helix as found in Er-1 and Er-10, and the simultaneous presence of 2 different conformations for the C-terminal tetrapeptide segment, i.e., a major conformation with the Leu 39-Pro 40 peptide bond in the trans form and a minor conformation with this peptide bond in the cis form.
About this Structure
1ERD is a Single protein structure of sequence from Euplotes raikovi. Full crystallographic information is available from OCA.
Reference
The NMR solution structure of the pheromone Er-2 from the ciliated protozoan Euplotes raikovi., Ottiger M, Szyperski T, Luginbuhl P, Ortenzi C, Luporini P, Bradshaw RA, Wuthrich K, Protein Sci. 1994 Sep;3(9):1515-26. PMID:7833811 Page seeded by OCA on Fri May 2 15:25:58 2008
