2v9b

From Proteopedia

(Difference between revisions)

Revision as of 10:43, 7 July 2021

X-ray structure of viscotoxin B2 from Viscum album

Structural highlights

2v9b is a 2 chain structure with sequence from Viscum album. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1jmp, 1orl
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[THNB_VISAL] Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.[REFERENCE:3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Crystals of the cytotoxic thionin proteins viscotoxins A1 and B2 extracted from mistletoe diffracted to high resolution (1.25 and 1.05 A, respectively) and are excellent candidates for testing crystallographic methods. Ab initio direct methods were only successful in solving the viscotoxin B2 structure, which with 861 unique non-H atoms is one of the largest unknown structures without an atom heavier than sulfur to be solved in this way, but sulfur-SAD phasing provided a convincing solution for viscotoxin A1. Both proteins form dimers in the crystal and viscotoxin B2 (net charge +4 per monomer), but not viscotoxin A1 (net charge +6), is coordinated by sulfate or phosphate anions. The viscotoxin A1 crystal has a higher solvent content than the viscotoxin B2 crystal (49% as opposed to 28%) with solvent channels along the crystallographic 4(3) axes.

Structures of viscotoxins A1 and B2 from European mistletoe solved using native data alone.,Pal A, Debreczeni JE, Sevvana M, Gruene T, Kahle B, Zeeck A, Sheldrick GM Acta Crystallogr D Biol Crystallogr. 2008 Sep;64(Pt 9):985-92. Epub 2008, Aug 13. PMID:18703848^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pal A, Debreczeni JE, Sevvana M, Gruene T, Kahle B, Zeeck A, Sheldrick GM. Structures of viscotoxins A1 and B2 from European mistletoe solved using native data alone. Acta Crystallogr D Biol Crystallogr. 2008 Sep;64(Pt 9):985-92. Epub 2008, Aug 13. PMID:18703848 doi:10.1107/S0907444908022646

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2v9b"

@@ Line 3: / Line 3: @@
 <StructureSection load='2v9b' size='340' side='right'caption='[[2v9b]], [[Resolution|resolution]] 1.05&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2v9b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V9B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V9B FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2v9b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V9B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V9B FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1jmp|1jmp]], [[1orl|1orl]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1jmp|1jmp]], [[1orl|1orl]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v9b OCA], [http://pdbe.org/2v9b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v9b RCSB], [http://www.ebi.ac.uk/pdbsum/2v9b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v9b ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v9b OCA], [https://pdbe.org/2v9b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v9b RCSB], [https://www.ebi.ac.uk/pdbsum/2v9b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v9b ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/THNB_VISAL THNB_VISAL]] Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.[REFERENCE:3]
+[[https://www.uniprot.org/uniprot/THNB_VISAL THNB_VISAL]] Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.[REFERENCE:3]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

2v9b

From Proteopedia

Revision as of 10:43, 7 July 2021

X-ray structure of viscotoxin B2 from Viscum album

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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