6vou
From Proteopedia
(Difference between revisions)
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==Aminoglycoside N-2'-Acetyltransferase-Ia [AAC(2')-Ia] in complex with acetylated-plazomicin and CoA== | ==Aminoglycoside N-2'-Acetyltransferase-Ia [AAC(2')-Ia] in complex with acetylated-plazomicin and CoA== | ||
| - | <StructureSection load='6vou' size='340' side='right'caption='[[6vou]]' scene=''> | + | <StructureSection load='6vou' size='340' side='right'caption='[[6vou]], [[Resolution|resolution]] 1.95Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VOU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6vou]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"proteus_stuartii"_buttiaux_et_al._1954 "proteus stuartii" buttiaux et al. 1954]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VOU FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vou OCA], [https://pdbe.org/6vou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vou RCSB], [https://www.ebi.ac.uk/pdbsum/6vou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vou ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PZC:(2S)-N-[(1R,2S,3S,4R,5S)-4-{[(2S,3R)-3-(acetylamino)-6-{[(2-hydroxyethyl)amino]methyl}-3,4-dihydro-2H-pyran-2-yl]oxy}-5-amino-2-{[3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranosyl]oxy}-3-hydroxycyclohexyl]-4-amino-2-hydroxybutanamide'>PZC</scene>, <scene name='pdbligand=TCE:3,3,3-PHOSPHANETRIYLTRIPROPANOIC+ACID'>TCE</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6vr3|6vr3]], [[6vr2|6vr2]], [[6vta|6vta]]</div></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aac ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=588 "Proteus stuartii" Buttiaux et al. 1954])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Gentamicin_2'-N-acetyltransferase Gentamicin 2'-N-acetyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.59 2.3.1.59] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vou OCA], [https://pdbe.org/6vou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vou RCSB], [https://www.ebi.ac.uk/pdbsum/6vou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vou ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The approval of plazomicin broadened the clinical library of aminoglycosides available for use against emerging bacterial pathogens. Contrarily to other aminoglycosides, resistance to plazomicin is limited; still, instances of resistance have been reported in clinical settings. Here, we present structural insights into the mechanism of plazomicin action and the mechanisms of clinical resistance. The structural data reveal that plazomicin exclusively binds to the 16S ribosomal A site, where it likely interferes with the fidelity of mRNA translation. The unique extensions to the core aminoglycoside scaffold incorporated into the structure of plazomicin do not interfere with ribosome binding, which is analogously seen in the binding of this antibiotic to the AAC(2')-Ia resistance enzyme. The data provides a structural rationale for resistance conferred by drug acetylation and ribosome methylation, i.e., the two mechanisms of resistance observed clinically. Finally, the crystal structures of plazomicin in complex with both its target and the clinically relevant resistance factor provide a roadmap for next-generation drug development that aims to ameliorate the impact of antibiotic resistance. | ||
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| + | Structural basis for plazomicin antibiotic action and resistance.,Golkar T, Bassenden AV, Maiti K, Arya DP, Schmeing TM, Berghuis AM Commun Biol. 2021 Jun 11;4(1):729. doi: 10.1038/s42003-021-02261-4. PMID:34117352<ref>PMID:34117352</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6vou" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Proteus stuartii buttiaux et al. 1954]] | ||
| + | [[Category: Gentamicin 2'-N-acetyltransferase]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Bassenden | + | [[Category: Bassenden, A V]] |
| - | [[Category: Berghuis | + | [[Category: Berghuis, A M]] |
| + | [[Category: Acetyltransferase]] | ||
| + | [[Category: Gnat superfamily]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 09:59, 14 July 2021
Aminoglycoside N-2'-Acetyltransferase-Ia [AAC(2')-Ia] in complex with acetylated-plazomicin and CoA
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