7ax0
From Proteopedia
(Difference between revisions)
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==Crystal structure of the computationally designed Scone-E protein co-crystallized with STA form a== | ==Crystal structure of the computationally designed Scone-E protein co-crystallized with STA form a== | ||
| - | <StructureSection load='7ax0' size='340' side='right'caption='[[7ax0]]' scene=''> | + | <StructureSection load='7ax0' size='340' side='right'caption='[[7ax0]], [[Resolution|resolution]] 2.20Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AX0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7ax0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AX0 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ax0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ax0 OCA], [https://pdbe.org/7ax0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ax0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ax0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ax0 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SIW:Keggin+(STA)'>SIW</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ax0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ax0 OCA], [https://pdbe.org/7ax0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ax0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ax0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ax0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Recent years have seen an increase in the development of computational proteins, including symmetric ones. A ninefold-symmetric beta-propeller protein named Cake has recently been developed. Here, attempts were made to further engineer this protein into a threefold-symmetric nine-bladed propeller using computational design. Two nine-bladed propeller proteins were designed, named Scone-E and Scone-R. Crystallography, however, revealed the structure of both designs to adopt an eightfold conformation with distorted termini, leading to a pseudo-symmetric protein. One of the proteins could only be crystallized upon the addition of a polyoxometalate, highlighting the usefulness of these molecules as crystallization additives. | ||
| + | |||
| + | Crystal structures of Scone: pseudosymmetric folding of a symmetric designer protein.,Mylemans B, Killian T, Vandebroek L, Van Meervelt L, Tame JRH, Parac-Vogt TN, Voet ARD Acta Crystallogr D Struct Biol. 2021 Jul 1;77(Pt 7):933-942. doi:, 10.1107/S2059798321005787. Epub 2021 Jun 18. PMID:34196619<ref>PMID:34196619</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ax0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Mylemans B]] | + | [[Category: Synthetic construct sequences]] |
| - | [[Category: Parac-Vogt | + | [[Category: Mylemans, B]] |
| - | [[Category: Vandebroek L]] | + | [[Category: Parac-Vogt, T N]] |
| - | [[Category: Voet | + | [[Category: Vandebroek, L]] |
| + | [[Category: Voet, A R.D]] | ||
| + | [[Category: Beta-propeller]] | ||
| + | [[Category: Computational design]] | ||
| + | [[Category: De novo protein]] | ||
Revision as of 10:04, 14 July 2021
Crystal structure of the computationally designed Scone-E protein co-crystallized with STA form a
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